IRS1 antibody (pSer307)

Catalog No. ABIN129580

Supplier Product No.: 600-401-445

Product Details anti-IRS1 Antibody

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Binding Specificity: pSer307

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Supplier Product No.: 600-401-445

Supplier: Rockland

Purpose: IRS1 phospho S307 Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against the phosphorylated form of human IRS1 protein at the pS307 residue.

Characteristics: Synonyms: rabbit anti-IRS1 pS307 antibody, IRS-1, IRS 1, HIRS 1 antibody, HIRS-1 antibody, Insulin Receptor Substrate 1 antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 290-320 of human IRS1 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to phosphorylated IRS1 protein by western blotting in the appropriate cell lysate or extract.   Minimal reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for pS307 of IRS1 protein.

Western Blot Dilution: 1:250 - 1:1,500

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.76 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-IRS1 antibody (ABIN129580)

Shah, Hunter: "Turnover of the active fraction of IRS1 involves raptor-mTOR- and S6K1-dependent serine phosphorylation in cell culture models of tuberous sclerosis." in: Molecular and cellular biology, Vol. 26, Issue 17, pp. 6425-34, (2006) (PubMed).

Target Details for IRS1

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Alternative Name: IRS1 (IRS1 Products)

Background: Background: Insulin Receptor Substrate 1 (IRS1) acts as a signaling molecule for IL-4, insulin and insulin-like growth factor-I (IGF-I) receptors.  When phosphorylated by the insulin receptor, IRS1 binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2.  When bound, IRS1 typically activates phosphatidylinositol 3-kinase p85 subunit.  IRS1 interacts with both the NPXY motif of tyrosine-phosphorylated IGF1R and the INSR through the PTB domain.  Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Diseases associated with IRS1 include Diabetes Mellitus, Noninsulin-Dependent and Mixed Cell Adenoma. Anti-IRS1pS307 is useful for researchers interested in protein kinase binding, IL-2 pathways, and RET signaling.

Gene ID: 3667

NCBI Accession: NP_005535

UniProt: P35568

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process