V5 Epitope Tag antibody

Catalog No. ABIN129577

Product Details anti-V5 Epitope Tag Antibody

Target: V5 Epitope Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This V5 Epitope Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Sequence: GKPIPNPLLG LDST

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This antibody was purified from whole rabbit serum prepared by repeated immunizations with V5 epitope tag peptide corresponding to aa 95-108 of the V protein conjugated to KLH using maleimide.

Isotype: IgG

Application Details

Application Notes: Anti-V5 is optimally suited for monitoring expression of V5-tagged fusion proteins. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). This antibody has been tested by ELISA and western blotting against both the immunizing peptide and V5 containing recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.73 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-V5 Epitope Tag antibody (ABIN129577)

Kotredes, Razmpour, Lutton, Alfonso-Prieto, Ramirez, Gamero: "Characterization of cancer-associated IDH2 mutations that differ in tumorigenicity, chemosensitivity and 2-hydroxyglutarate production." in: Oncotarget, Vol. 10, Issue 28, pp. 2675-2692, (2019) (PubMed).

Schweiger, Clement, Page, Schepeler, Zou, Sirokmány, Watt, Jensen: "Lrig1 marks a population of gastric epithelial cells capable of long-term tissue maintenance and growth in vitro." in: Scientific reports, Vol. 8, Issue 1, pp. 15255, (2018) (PubMed).

Glaeser, Urban, Fenech, Voloshanenko, Kranz, Lari, Christianson, Boutros: "ERAD-dependent control of the Wnt secretory factor Evi." in: The EMBO journal, Vol. 37, Issue 4, (2018) (PubMed).

Watanabe, Simon, Durham, Kemp, Machihara, Kemal, Shi, Foley, Li, Chen, Weiser, Burger, Anastos, Chen, Carter: "The HIV-1 late domain-2 S40A polymorphism in antiretroviral (or ART)-exposed individuals influences protease inhibitor susceptibility." in: Retrovirology, Vol. 13, Issue 1, pp. 64, (2017) (PubMed).

Huang, Li, Chen: "Flexible catalytic site conformations implicated in modulation of HIV-1 protease autoprocessing reactions." in: Retrovirology, Vol. 8, Issue 1, pp. 79, (2012) (PubMed).

Target Details for V5 Epitope Tag

Target: V5 Epitope Tag

Alternative Name: V5-Tag (V5 Epitope Tag Products)

Target Type: Tag

Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  Supplier produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG, HA and V5. Supplier also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.

Gene ID: 55775699