Phosphoserine antibody

Catalog No. ABIN129572

Supplier Product No.: 600-401-261

Product Details anti-Phosphoserine Antibody

Target: Phosphoserine

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phosphoserine antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Supplier Product No.: 600-401-261

Supplier: Rockland

Purpose: Phosphoserine Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit IgG.

Characteristics: Synonyms: pS antibody, phospho serine AB, PhosphoSerine (pS) reactive antibody, proteinogenic amino acid antibodies, Phosphoserine [pS] polyclonal antibody, PhosphoSerine antibody produced in rabbit

Purification: Anti-Phosphoserine Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phosphorylated serine peptide coupled to agarose.

Sterility: Sterile filtered

Immunogen:

Immunogen: Phosphoserine Antibody was prepared from whole rabbit serum produced by repeated immunizations with phosphoserine conjugated KLH.

Immunogen Type: Other

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: User Optimized

Application Note: Phosphoserine Antibody is tested for use in ELISA, western blotting, Immunohistochemistry-P, and immunoprecipitation assays.  The antibody reacts specifically with phosphoserine and shows minimal reactivity by ELISA and western blotting with proteins containing phosphotyrosine or phosphothreonine residues.  The antibody reacts with free phosphoserine, phosphoserine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphoserine in proteins of both unstimulated and stimulated cell lysates.  Although not tested, this antibody is likely functional in RIA and flow cytometry.

Western Blot Dilution: 1:500-1:1,000

Immunoprecipitation Dilution: 10 μg/mg protein sample

ELISA Dilution: 1:1,000-1:2,500

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-Phosphoserine antibody (ABIN129572)

Cohen, Fernandez, Lázaro-Diéguez, Müsch: "The serine/threonine kinase Par1b regulates epithelial lumen polarity via IRSp53-mediated cell-ECM signaling." in: The Journal of cell biology, Vol. 192, Issue 3, pp. 525-40, (2011) (PubMed).

Target Details for Phosphoserine

Target: Phosphoserine

Abstract: Phosphoserine Products

Target Type: Amino Acid

Background: Background: Anti-Phosphoserine Antibody detects phosphorylated Serines. Serine is one of the proteinogenic amino acids. By virtue of the hydroxyl group, serine is classified as a polar amino acid. It is not essential to the human diet, since it is synthesized in the body from other metabolites, including glycine. Serine is important in metabolism in that it participates in the biosynthesis of purines and pyrimidines. It is the precursor to several amino acids including glycine and cysteine, and tryptophan in bacteria. It is also the precursor to numerous other metabolites, including sphingolipids and folate, which is the principal donor of one-carbon fragments in biosynthesis. Anti-phospho Serine Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Immunology, Cancer research and Signal Transduction research.