RAD23B antibody (AA 155-180)

Catalog No. ABIN129561

Supplier Product No.: 600-101-391

Product Details anti-RAD23B Antibody

Target: RAD23B (RAD23 Homolog B (RAD23B))

Binding Specificity: AA 155-180

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RAD23B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-101-391

Supplier: Rockland

Purpose: HR23B Antibody

Cross-Reactivity (Details): Reactivity occurs against human HR23B protein.

Characteristics: Synonyms: goat anti-HR23B antibody, hHR23B antibody, mHR23B antibody, p58 antibody, RAD23 (S. cerevisiae) homolog B antibody, UV excision repair protein RAD23 homolog B, XP-C repair-complementing complex 58 kDa protein

Purification: This is an affinity-purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 155-180 of human HR23B protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 58 kDa in size corresponding to HR23B by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:2,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-RAD23B antibody (ABIN129561)

Crosby, Kulshreshtha, Ivan, Glazer: "MicroRNA regulation of DNA repair gene expression in hypoxic stress." in: Cancer research, Vol. 69, Issue 3, pp. 1221-9, (2009) (PubMed).

Shen, Valencia, Szostak, Szostak, Dong, Liu: "Scanning the human proteome for calmodulin-binding proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 17, pp. 5969-74, (2005) (PubMed).

Target Details for RAD23B

Target: RAD23B (RAD23 Homolog B (RAD23B))

Alternative Name: RAD23B (RAD23B Products)

Background: Background: HR23B (also known as UV excision repair protein RAD23 homolog B, XP-C repair complementing complex 58 kDa protein and p58) is one of two human homologs of Saccharomyces cerevisiae Rad23 (hHR23A and hHR23B), a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

Gene ID: 5887, 4506387

UniProt: P54727

Pathways: DNA Damage Repair