UBE2N antibody (AA 30-55)

Catalog No. ABIN129560

Supplier Product No.: 600-101-342

Product Details anti-UBE2N Antibody

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Binding Specificity: AA 30-55

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This UBE2N antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-101-342

Supplier: Rockland

Purpose: UBC13 Antibody

Cross-Reactivity (Details): This affinity-purified antibody is directed against human UBC13 protein.

Characteristics: Synonyms: goat anti-UBC13 antibody, UBEN2, BLU, Ubch13, UBC 13, UbCH ben antibody, Ube 2N antibody, ubiquitin carrier protein N antibody, Ubiquitin conjugating enzyme E2N antibody, ubiquitin protein ligase N antibody, Ubiquitin-conjugating enzyme E2 N antibody

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-55 of human UBC13 protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 17 kDa in size corresponding to UBC13 protein by western blotting in the appropriate tissue or cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.25 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for UBE2N

Target: UBE2N (Ubiquitin-Conjugating Enzyme E2N (UBE2N))

Alternative Name: UBC13 (UBE2N Products)

Background: Background: UBC13 is a member of the E2 ubiquitin-conjugating enzyme family also known as Ubiquitin-conjugating enzyme E2 N, Ubiquitin-protein ligase N, Ubiquitin carrier protein N and Bendless-like ubiquitin-conjugating enzyme.  The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. UBC13 plays a role in cell cycle, differentiation, cell survival, and DNA repair. Anti-UBC13 Antibody is useful for researchers interested in Interferon gamma signaling, TLR4 signaling, RND binding, and ubiquitin protein activities.

Gene ID: 7334, 4507793

UniProt: P61088

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Positive Regulation of Response to DNA Damage Stimulus, Ubiquitin Proteasome Pathway