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POLK antibody (AA 817-830)

Rockland 600-101-295 POLK Reactivity: Human WB, ELISA Host: Goat Polyclonal unconjugated
Rockland
Catalog No. ABIN129559
Supplier Product No.: 600-101-295
  • Target See all POLK Antibodies
    POLK (Polymerase (DNA Directed) kappa (POLK))
    Binding Specificity
    • 15
    • 7
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 817-830
    Reactivity
    • 54
    • 15
    • 13
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 1
    • 1
    Human
    Host
    • 47
    • 5
    • 2
    Goat
    Clonality
    • 51
    • 3
    Polyclonal
    Conjugate
    • 25
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This POLK antibody is un-conjugated
    Application
    • 28
    • 19
    • 14
    • 5
    • 4
    • 4
    • 2
    • 1
    Western Blotting (WB), ELISA
    Supplier Product No.
    600-101-295
    Supplier
    Rockland
    Purpose
    POLK Antibody
    Cross-Reactivity (Details)
    This affinity purified antibody is directed against human POLK protein.
    Characteristics
    Synonyms: goat Anti-POLK antibody, goat Anti-DNA polymerase kappa antibody, DINB homolog 1 (E. coli) antibody, DINB protein antibody, DINB1 antibody, DINP antibody, DNA damage inducible protein b antibody, POLQ antibody
    Purification
    The product was affinity purified from monospecific antiserum by immunoaffinity purification.
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: This affinity-purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 817-830 of Human POLK (polymerase (DNA directed) kappa).

    Immunogen Type: Conjugated Peptide

    Isotype
    IgG
    Top Product
    Discover our top product POLK Primary Antibody
  • Application Notes

    Application Note: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 98.8 kDa in size corresponding to POLK by western blotting in the appropriate cell lysate or extract.  Splice variants for this protein may show molecular weights of 89.0 and 76.4 kDa.

    Western Blot Dilution: 1:500 - 1:3,000

    ELISA Dilution: 1:2,000 - 1:10,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.4 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    POLK (Polymerase (DNA Directed) kappa (POLK))
    Alternative Name
    POLK (POLK Products)
    Background
    Background: POLK (also known as DNA polymerase kappa, DINB protein and DINP) is involved in DNA repair.  POLK plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. POLK lacks 3'-5' proofreading exonuclease activity.  POLK is a nuclear protein that binds to REV1L and PCNA. POLK is detected throughout the nucleus and at replication foci. Multiple alternative splice variants have been detected for this protein. POLK is found at low levels in testis, spleen, prostate and ovary and at very low levels in kidney, colon, brain, heart, liver, lung, placenta, pancreas and peripheral blood leukocytes.
    Gene ID
    51426, 59798438
    UniProt
    Q9UBT6
    Pathways
    DNA Damage Repair
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