DCLRE1C antibody (Internal Region)

Catalog No. ABIN129558

Supplier Product No.: 600-101-293

Product Details anti-DCLRE1C Antibody

Target: DCLRE1C (DNA Cross-Link Repair 1C (DCLRE1C))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This DCLRE1C antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 600-101-293

Supplier: Rockland

Purpose: ARTEMIS Antibody

Cross-Reactivity (Details): This antibody detects a band of the appropriate size in a number of different cell lines.

Characteristics: Synonyms: goat anti-Artemis Antibody, A-SCID antibody, A SCID protein antibody, protein Artemis antibody, DCLRE1C antibody, DCLRE1C, DNA cross link repair 1C antibody, ASCID, SCIDA, SNM1C

Purification: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding an internal region of Human ARTEMIS (DCLRE1C DNA cross-link repair 1C).

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000

Application Note: This affinity purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting.  Reactivity in other immunoassays is unknown. This antibody detects a band of approximately 90 kDa (predicted molecular weight: 78 kDa).

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:10,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.2 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for DCLRE1C

Target: DCLRE1C (DNA Cross-Link Repair 1C (DCLRE1C))

Alternative Name: DCLRE1C (DCLRE1C Products)

Background: Background: Artemis has a role in T and B lymphocyte immunodeficiency and in predisposition to lymphoma through the NHEJ pathway of DNA repair. DNA-PKcs regulates Artemis by both phosphorylation and complex formation to permit enzymatic activities that are critical for the hairpin-opening step of V(D)J recombination and for the 5' and 3' overhang processing in non-homologous DNA end joining.

Gene ID: 64421, 76496495

UniProt: Q96SD1

Pathways: DNA Damage Repair