RAD23A antibody (AA 115-140)

Catalog No. ABIN129556

Supplier Product No.: 600-101-291

Product Details anti-RAD23A Antibody

Target: RAD23A (RAD23 Homolog A (RAD23A))

Binding Specificity: AA 115-140

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This RAD23A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-101-291

Supplier: Rockland

Purpose: HR23A Antibody

Cross-Reactivity (Details): Reactivity occurs against human HR23A protein.

Characteristics: Synonyms: goat anti-HR23A Antibody, hHR23A antibody, MGC111083 antibody, RAD23 homolog A antibody, RAD23 yeast homolog A antibody, RAD23A antibody, UV excision repair protein RAD23 homolog A

Purification: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 115-140 of human HR23A protein.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 60 kDa in size corresponding to HR23A by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:4,000 - 1:16,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.2 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-RAD23A antibody (ABIN129556)

Zhu, Wani, Arab, El-Mahdy, Ray, Wani: "Chromatin restoration following nucleotide excision repair involves the incorporation of ubiquitinated H2A at damaged genomic sites." in: DNA repair, Vol. 8, Issue 2, pp. 262-73, (2009) (PubMed).

Target Details for RAD23A

Target: RAD23A (RAD23 Homolog A (RAD23A))

Alternative Name: RAD23A (RAD23A Products)

Background: Background: HR23A (also known as UV excision repair protein RAD23 homolog A) is one of two human homologs of Saccharomyces cerevisiae Rad23 (hHR23A and hHR23B), a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.

Gene ID: 5886, 4826964

UniProt: P54725

Pathways: DNA Damage Repair