ING5 antibody (AA 125-150)

Catalog No. ABIN129552

Supplier Product No.: 600-101-285

Product Details anti-ING5 Antibody

Target: ING5 (Inhibitor of Growth Family, Member 5 (ING5))

Binding Specificity: AA 125-150

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ING5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-101-285

Supplier: Rockland

Purpose: p28ING5 Antibody

Cross-Reactivity (Details): Reactivity occurs against human p28 ING5 protein.

Characteristics: Synonyms: goat anti-p28 ING5 Antibody, p28ING5, ING-5 antibody, ING 5, Inhibitor of growth family member 5 antibody

Purification: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 125-150 of Human p28 ING5 protein (Inhibitor of growth family, member 5).

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 28 kDa or 26 kDa in size corresponding to variants of ING5 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:1,000

ELISA Dilution: 1:4,000 - 1:16,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.52 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-ING5 antibody (ABIN129552)

Iizuka, Sarmento, Sekiya, Scrable, Allis, Smith: "Hbo1 Links p53-dependent stress signaling to DNA replication licensing." in: Molecular and cellular biology, Vol. 28, Issue 1, pp. 140-53, (2007) (PubMed).

Target Details for ING5

Target: ING5 (Inhibitor of Growth Family, Member 5 (ING5))

Alternative Name: ING5 (ING5 Products)

Background: Background: p28 ING5 is a tumor suppressor protein similar to ING1 that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in TP53-dependent regulatory pathway.  Multiple alternatively spliced transcript variants have been observed.  The accession number listed below is for variant (1) encodes the longest isoform.

Gene ID: 84289, 18644730

UniProt: Q8WYH8

Pathways: Chromatin Binding