ING1 antibody (AA 275-300)

Catalog No. ABIN129550

Supplier Product No.: 600-101-282

Product Details anti-ING1 Antibody

Target: ING1 (Inhibitor of Growth Family, Member 1 (ING1))

Binding Specificity: AA 275-300

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ING1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 600-101-282

Supplier: Rockland

Purpose: p33ING1 Antibody

Cross-Reactivity (Details): Reactivity occurs against human p33 ING1 protein and is 100 % homologous to all splice variants for ING1.

Characteristics: Synonyms: goat anti-p33 ING1 antibody, p33ING1, ING-1, ING 1, Growth inhibitory protein ING1 antibody, Homo sapiens growth inhibitor p33ING1 (ING1) mRNA, complete cds antibody, ING 1 antibody, Inhibitor of growth 1 antibody, Inhibitor of growth family member 1 antibody

Purification: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole Goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 275-300 of Human p33 ING1 protein (Inhibitor of growth family, member 1). 

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity-purified antibody has been tested for use in ELISA and western blotting using over expressed cell lysates. Expect a band approximately 47 kDa, 33 kDa or 24 kDa in size corresponding to variants of ING1 by western blotting in the appropriate cell lysate or extract. Specific conditions for other applications should be optimized by the end user.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:4,000 - 1:14,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ING1

Target: ING1 (Inhibitor of Growth Family, Member 1 (ING1))

Alternative Name: ING1 (ING1 Products)

Background: Background: p33 ING1 is a tumor suppressor protein that can induce cell growth arrest and apoptosis. The encoded protein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53-signaling pathway. Reduced expression and rearrangement of this gene have been detected in various cancers. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported.  The accession number listed below is for variant (4) that encodes the longest isoform (D).   Other synonyms for p33 ING1 include p33, p47, p33ING1, p24ING1c, p33ING1b, p47ING1a, growth inhibitor ING1, inhibitor of growth 1, tumor suppressor ING1 and growth inhibitory protein ING1.

Gene ID: 3621, 38201661

UniProt: Q9UK53

Pathways: Protein targeting to Nucleus, Autophagy