MTBP antibody (AA 667-812)

Catalog No. ABIN129549

Supplier Product No.: 500-301-875

Product Details anti-MTBP Antibody

Target: MTBP (MDM2 Binding Protein (MTBP))

Binding Specificity: AA 667-812

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MTBP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 500-301-875

Supplier: Rockland

Purpose: MTBP Antibody

Cross-Reactivity (Details): This antibody is directed against human MTBP protein.

Characteristics: Synonyms: mouse anti-MTBP antibody, mouse anti-mouse double minute 2 antibody, MDM 2 BP antibody, MDM2 (mouse double minute 2) binding protein 104kD antibody, MDM2 (mouse double minute 2) binding protein antibody, MDM2 Binding Protein antibody, MDM2 BP antibody

Purification: The product was produced from clarified mouse ascetic fluid.

Sterility: Sterile filtered

Immunogen:

Immunogen: This monoclonal antibody was produced by repeated immunizations with a recombinant amino-terminal HIS_6X-tagged polypeptide corresponding to amino acid residues 667-812 of human MTBP protein.

Immunogen Type: Conjugated Peptide

Clone: PHL-1

Isotype: IgG1 kappa

Application Details

Application Notes:

Application Note: This antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 110 kDa in size corresponding to MTBP by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:200 - 1:2,000

ELISA Dilution: 1:2,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MTBP

Target: MTBP (MDM2 Binding Protein (MTBP))

Alternative Name: MTBP (MTBP Products)

Background: Background: The MDM2 onco-protein is over expressed in human cancer cells and it promotes tumorigenesis by directly blocking the function of the p53 tumor suppressor.  Studies also indicate that MDM2 regulates cell proliferation via p53 independent mechanisms.   MTBP (Mdm2 [Two] Binding Protein) was identified by a yeast two-hybrid screen as an MDM2 binding protein (1).  MTBP has been reported to modulate cell cycle progression by a p53 independent mechanism and suppress MDM2-mediated p53 degradation when ectopically expressed in cells (1,2).   The human MTBP gene localizes to 8q24 (3) and MTBP RNA is expressed in a wide range of tissues, with highest being in the thymus, testes and ovaries (1).

Gene ID: 27085

NCBI Accession: NP_071328

UniProt: Q96DY7