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SARS-CoV-2 NSP5 (3CL-Pro) antibody

3CL-PRO, M-Pro Reactivity: SARS Coronavirus (SARS-CoV) ELISA, WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN129545
  • Target See all SARS-CoV-2 NSP5 (3CL-Pro) (3CL-PRO, M-Pro) Antibodies
    SARS-CoV-2 NSP5 (3CL-Pro) (3CL-PRO, M-Pro) (SARS-CoV-2 3C-Like Proteinase (NSP5) (3CL-PRO, M-Pro))
    Reactivity
    SARS Coronavirus (SARS-CoV)
    Host
    • 3
    • 1
    Rabbit
    Clonality
    • 4
    Polyclonal
    Conjugate
    • 4
    This SARS-CoV-2 NSP5 (3CL-Pro) antibody is un-conjugated
    Application
    • 3
    • 3
    • 1
    ELISA, Western Blotting (WB)
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Immunogen
    This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant protein corresponding to full length SARS Coronavirus 3CL Protease. Lifesensors Inc. prepared the 3CL Protease as follows: SUMO-3CL protease fusion was expressed in E. coli in LB medium and purified by Ni-NTA resin affinity chromatography (Qiagen). After the fusion was cleaved by the SUMO Protease (LifeSensors), the SUMO tag and protease were subtracted from the 3CL protease using MAC and the 3CL protease was finally purified using Anion Exchange Chromatography with the Macro-Prep High Q resin (BioRad) and size exclusion chromatography.
    Immunogentype:Recombinant
    Isotype
    IgG
    Top Product
    Discover our top product 3CL-PRO, M-Pro Primary Antibody
  • Application Notes
    This protein A purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 34 kDa in size corresponding to SARS 3CL Protease by western blotting in the appropriate cell lysate or extract.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Restore with deionized water (or equivalent)
    Concentration
    5.0 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
  • Target
    SARS-CoV-2 NSP5 (3CL-Pro) (3CL-PRO, M-Pro) (SARS-CoV-2 3C-Like Proteinase (NSP5) (3CL-PRO, M-Pro))
    Alternative Name
    SARS 3Cl Protease (3CL-PRO, M-Pro Products)
    Synonyms
    pp1ab antibody, ORF1a polyprotein;ORF1ab polyprotein antibody, ORF1ab antibody
    Target Type
    Viral Protein
    Background
    Generally, viruses have proteases to process their proteins into active form. Because of its pivotal role in the viral life cycle, proteases are primary targets for the development of antiviral agents. 3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase. 3CL protease is the only Coronavirus protein for which structural information is available. 3CL protease comprises three domains, the substrate-binding site is expected to be located between domains I and II, and domain III is a globular cluster comprising five helices. 3CL protease is a homodimer.
    Synonyms: 3CL PRO antibody, 3CLp antibody, nsp5 antibody
    Gene ID
    1489680, 29837498
    UniProt
    P0C6U8
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