RFFL antibody (AA 1-363)

Catalog No. ABIN129535

Product Details anti-RFFL Antibody

Target: RFFL (Ring Finger and FYVE-Like Domain Containing 1 (RFFL))

Binding Specificity: AA 1-363

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFFL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-363 of human RFFL protein.
Immunogentype:Recombinant

Isotype: IgG

Application Details

Application Notes: This protein A purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kDa in size corresponding to RFFL protein by western blotting in the appropriate tissue or cell lysate or extract.  Isoforms 2 and 3 are 39.7 and 36.6 kDa, respectively and should also cross react with this antibody.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 5.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for RFFL

Target: RFFL (Ring Finger and FYVE-Like Domain Containing 1 (RFFL))

Alternative Name: RFFL (RFFL Products)

Synonyms: MGC84042 antibody, RFFL antibody, Rffl antibody, 1700051E09Rik antibody, 4930516L10Rik antibody, BG080975 antibody, Carp2 antibody, CARP-2 antibody, CARP2 antibody, FRING antibody, RIFIFYLIN antibody, RNF189 antibody, RNF34L antibody, ring finger and FYVE like domain containing E3 ubiquitin protein ligase antibody, ring finger and FYVE-like domain containing E3 ubiquitin protein ligase L homeolog antibody, ring finger and FYVE-like domain containing 1 antibody, ring finger and FYVE like domain containing protein antibody, ring finger and FYVE-like domain containing E3 ubiquitin protein ligase antibody, RFFL antibody, rffl.L antibody, Rffl antibody

Background: This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. RFFL, also known as Rififylin, RING finger and FYVE-like domain-containing protein 1, FYVE-RING finger protein, Sakura, Fring, Caspases-8 and -10-associated RING finger protein 2, CARP-2, Caspase regulator CARP2, RING finger protein 189 and RING finger protein 34-like, is a novel modulator of NF-kB activation.  RFFL possesses E3 ubiquitin protein ligase activity and has been shown to regulate the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. RFFL also possesses anti-apoptotic activity and may bind phosphatidylinositol phosphates. RFFL is a membrane bound cytoplasmic protein that is expressed ubiquitously. RFFL can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes and is rapidly degraded after stimulation with TNFSF10, and probably by caspases. Multiple transcript variants have been detected for this protein.
Synonyms: RFFL, Rififylin, RING finger and FYVE-like domain-containing protein 1, FYVE-RING finger protein, Sakura, Fring, Caspases-8 and -10-associated RING finger protein 2, CARP-2, Caspase regulator CARP2, RING finger protein 189 and RING finger protein 34-like

Gene ID: 117584, 17432433

UniProt: Q8WZ73