RFFL antibody (AA 1-363)

Catalog No. ABIN129535

Supplier Product No.: 200-401-932

Product Details anti-RFFL Antibody

Target: RFFL (Ring Finger and FYVE-Like Domain Containing 1 (RFFL))

Binding Specificity: AA 1-363

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFFL antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 200-401-932

Supplier: Rockland

Purpose: RFFL Antibody

Cross-Reactivity (Details): This protein A purified antibody is directed against human RFFL protein.

Characteristics: Synonyms: rabbit anti-RFFL antibody, Rififylin, RING finger and FYVE-like domain-containing protein 1, FYVE-RING finger protein, Sakura, Fring, Caspases-8 and -10-associated RING finger protein 2, CARP-2, Caspase regulator CARP2, RING finger protein 189 and RING finger protein 34-like

Purification: The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer stated above.

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-363 of human RFFL protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:3,000

Application Note: This protein A purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kDa in size corresponding to RFFL protein by western blotting in the appropriate tissue or cell lysate or extract.  Isoforms 2 and 3 are 39.7 and 36.6 kDa, respectively and should also cross react with this antibody.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:4,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 5.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for RFFL

Target: RFFL (Ring Finger and FYVE-Like Domain Containing 1 (RFFL))

Alternative Name: RFFL (RFFL Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI). RFFL, also known as Rififylin, RING finger and FYVE-like domain-containing protein 1, FYVE-RING finger protein, Sakura, Fring, Caspases-8 and -10-associated RING finger protein 2, CARP-2, Caspase regulator CARP2, RING finger protein 189 and RING finger protein 34-like, is a novel modulator of NF-kB activation.  RFFL possesses E3 ubiquitin protein ligase activity and has been shown to regulate the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. RFFL also possesses anti-apoptotic activity and may bind phosphatidylinositol phosphates. RFFL is a membrane bound cytoplasmic protein that is expressed ubiquitously. RFFL can be detected in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes and is rapidly degraded after stimulation with TNFSF10, and probably by caspases. Multiple transcript variants have been detected for this protein. Anti-RFFL Antibody is useful for researcher interested in Cancer, Immunology, transcription, and TNF-alpha/NF-kB research.

Gene ID: 117584, 17432433

UniProt: Q8WZ73