SPANXC antibody

Catalog No. ABIN129534

Supplier Product No.: 200-401-915

Product Details anti-SPANXC Antibody

Target: SPANXC (SPANX Family, Member C (SPANXC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SPANXC antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Supplier Product No.: 200-401-915

Supplier: Rockland

Purpose: SPANX-C Antibody

Cross-Reactivity (Details): This Protein A purified antibody is directed against human SPANX-C protein.

Characteristics: Synonyms: rabbit anti-SPANX-C antibody, Cancer-testis-associated protein CTp11 antibody, CTP11 antibody, Nuclear associated protein SPAN-Xc antibody, SPANX family member C antibody, Sperm protein associated with the nucleus on the X chromosome C, SPANX family member C1 antibody, SPANXC

Purification: The product was purified from monospecific antiserum by protein A chromatography followed by exhaustive dialysis against the buffer stated above.

Immunogen:

Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length recombinant human SPANX-C protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1.25-2.5 μg/mL

Application Note: This protein A purified antibody has been tested for use in ELISA, immunohistochemistry, and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15-20 kDa in size corresponding to SPANX family members by western blotting in the appropriate human tissue.  Most human cell lines are SPANX negative.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 5.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for SPANXC

Target: SPANXC (SPANX Family, Member C (SPANXC))

Alternative Name: SPANXC (SPANXC Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Human Sperm Proteins Associated with the Nucleus on X-chromosome (SPANX) are relatively low molecular weight cytoplasmic proteins found in testis and sperm.  Their expression in other tissues indicates malignancies. Family members are observed as proteins that range from 15 to 20 kDa.

Gene ID: 64663, 6977870

UniProt: Q9NY87