RREB1 antibody

Catalog No. ABIN129532

Product Details anti-RREB1 Antibody

Target: RREB1 (Ras Responsive Element Binding Protein 1 (RREB1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RREB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: Rreb1 Antibody

Cross-Reactivity (Details): Anti-RREB1 antibody is directed against the mouse RREB1 protein.

Characteristics: Synonyms: rabbit anti-RREB1 Antibody, rabbit anti-RREB 1 Antibody, FINB antibody, Finger protein in nuclear bodies antibody, LZ321 antibody, Raf responsive zinc finger protein LZ321 antibody, RAS responsive element binding protein 1 antibody

Purification: The product was Protein A purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: Anti-RREB1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to mouse RREB1 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This Protein A purified antibody has been tested for use in ELISA and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 90-180 kDa in size corresponding to RREB1 by western blotting in the appropriate cell lysate or extract.  Multiple bands seen in the above western blot may indicate cross-reactive isoforms or truncated protein products.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:2,000 - 1:15,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-RREB1 antibody (ABIN129532)

Flajollet, Poras, Carosella, Moreau: "RREB-1 is a transcriptional repressor of HLA-G." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 11, pp. 6948-59, (2009) (PubMed).

Kuppuswamy, Vijayalingam, Zhao, Zhou, Subramanian, Ryerse, Chinnadurai: "Role of the PLDLS-binding cleft region of CtBP1 in recruitment of core and auxiliary components of the corepressor complex." in: Molecular and cellular biology, Vol. 28, Issue 1, pp. 269-81, (2008) (PubMed).

Target Details for RREB1

Target: RREB1 (Ras Responsive Element Binding Protein 1 (RREB1))

Alternative Name: Rreb1 (RREB1 Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Neuroscience, and Signal Transduction research. RREB1 (also known as RAS-responsive element binding protein 1 and Raf responsive zinc finger protein) is a transcription factor that binds specifically to the distal RAS-responsive element (RRE) of gene promoters. May be involved in Ras/Raf-mediated cell differentiation by enhancing calcitonin expression.

Gene ID: 68750

UniProt: Q3UH06