UBA1 antibody

Catalog No. ABIN129529

Product Details anti-UBA1 Antibody

Target: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: Anti-Ubiquitin Activating Enzyme E1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to full length Human Ubiquitin Activating Enzyme E1.
Immunogentype:Recombinant

Isotype: IgG

Application Details

Application Notes: This purified antibody has been tested for use in ELISA, immunohistochemistry and western blot.  Specific conditions for reactivity should be optimized by the end user.  Expect a band at ~118 kDa in size corresponding to UBE1 by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Restore with deionized water (or equivalent)

Concentration: 5.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for UBA1

Target: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Alternative Name: Ubiquitin Activating Enzyme E1 (UBA1 Products)

Background: Ubiquitin Activating Enzyme (E1), also known as A1S9 and UBE1, is responsible for the first step in ubiquitin-protein isopeptide bond formation.  E1 catalyzes the activation of the C-terminal carboxyl group of ubiquitin by forming a high-energy thioester bond in an ATP-dependent manner. UBE1 is monomeric and contains two active sites within the E1 molecule, allowing it to bind two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.  Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR. Isoform 1 has a different 5' noncoding exon compared to isoform 2.  Both variants encode the same protein.
Synonyms: A1S9 antibody, A1S9 protein antibody, A1S9T and BN75 temperature sensitivity complementing antibody, A1S9T antibody, A1ST antibody, GXP 1 antibody

Gene ID: 7317, 23510338

UniProt: P22314