S100A1 antibody

Catalog No. ABIN129527

Supplier Product No.: 200-401-874

Product Details anti-S100A1 Antibody

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Reactivity: Human, Mouse, Rat, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This S100A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Supplier Product No.: 200-401-874

Supplier: Rockland

Purpose: S100 Protein Antibody

Cross-Reactivity (Details): This Protein A purified antibody is directed against bovine S100 protein.

Characteristics: Synonyms: rabbit anti-S100 Protein Antibody, S100 calcium-binding protein A1, S-100 protein subunit alpha, S-100 protein alpha chain

Purification: The product was purified from monospecific antiserum by Protein A chromatography.

Immunogen:

Immunogen: This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full-length bovine S100 protein (mixture of aa homodimers and ab heterodimers).

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:200 - 1:2,000

Application Note: This Protein A purified antibody has been tested for use in immunohistochemistry, and western blot and is suitable for ELISA.  Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 11 kDa in size corresponding to S100 monomer by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 5.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for S100A1

Target: S100A1 (S100 Calcium Binding Protein A1 (S100A1))

Alternative Name: S100A1 (S100A1 Products)

Background: Background: S-100 protein derived from brain tissue is an acidic calcium-binding protein with molecular weight of about 21 kDa. In human brain tissue S-100 protein is mainly presented as two isoforms - bb homodimer (S-100b) and ab heterodimer (S-100a). Because of its predominant location in astroglial cells S-100 protein can be used as a sensitive and reliable marker for central nervous system injury. Structural damage of glial cells causes leakage of S-100 protein into the extracellular matrix and into cerebrospinal fluid, further releasing into the bloodstream. Measurements of S-100 protein in patient serum samples are useful in monitoring of traumatic brain injury, ischemic brain damage after circulatory arrests, and in diagnosis and prognosis of clinical outcome in acute stroke.  Although predominant among the water-soluble brain proteins, S-100 is also found in a variety of other tissues. S-100 is an intracellular protein that weakly binds calcium. It binds zinc very tightly, however, and this appears to increase the affinity of the protein for calcium. Distinct binding sites, with different affinities, exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.

Gene ID: 528735

NCBI Accession: NP_001092512

UniProt: P02639

Pathways: Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades, S100 Proteins