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NRF1 antibody (AA 1-534)

NRF1 Reactivity: Human, Mouse WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN129526
  • Target See all NRF1 Antibodies
    NRF1 (Nuclear Respiratory Factor 1 (NRF1))
    Binding Specificity
    • 15
    • 9
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-534
    Reactivity
    • 68
    • 42
    • 36
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 64
    • 5
    Rabbit
    Clonality
    • 55
    • 14
    Polyclonal
    Conjugate
    • 37
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NRF1 antibody is un-conjugated
    Application
    • 56
    • 23
    • 14
    • 14
    • 13
    • 12
    • 7
    • 5
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Purpose
    NRF1 Antibody
    Cross-Reactivity (Details)
    This protein A purified antibody is directed against mouse NRF1.
    Characteristics
    Synonyms: rabbit anti-NRF1 antibody, rabbit anti-NRF 1 antibody, NRF-1, alpha pal antibody, alpha palindromic binding protein antibody, locus control region factor 1 antibody, NFE2 related factor 1 antibody, nuclear respiratory factor 1 antibody, transcription factor 11 antibody
    Purification
    The product was purified from monospecific antiserum by protein A affinity purification.
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant mouse NRF1 protein corresponding to aa 1- 534 of the native protein.

    Immunogen Type: Recombinant Protein

    Isotype
    IgG
    Top Product
    Discover our top product NRF1 Primary Antibody
  • Application Notes

    Immunohistochemistry Dilution: 2 mg/mL - 5 μg/mL

    Application Note: This protein A purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 67 kDa in size corresponding to NRF1 by western blotting in the appropriate cell lysate or extract.  Splice variants exist for this protein that may result in the detection of lower molecular weight bands.

    Western Blot Dilution: 1:500 - 1:2,000

    ELISA Dilution: 1:3,000 - 1:10,000

    Other: User Optimized

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    2.3 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Hinkley, Morton, Ichinoseki-Sekine, Huertas, Smuder: "Exercise Training Prevents Doxorubicin-induced Mitochondrial Dysfunction of the Liver." in: Medicine and science in sports and exercise, Vol. 51, Issue 6, pp. 1106-1115, (2019) (PubMed).

    Miura, Saitoh, Kokubun, Owada, Yamauchi, Machii, Takeishi: "Mitochondrial-Targeted Antioxidant Maintains Blood Flow, Mitochondrial Function, and Redox Balance in Old Mice Following Prolonged Limb Ischemia." in: International journal of molecular sciences, Vol. 18, Issue 9, (2018) (PubMed).

    Joseph, Nguyen, Welter, Dominguez, Behnke, Adhihetty: "Mitochondrial adaptations evoked with exercise are associated with a reduction in age-induced testicular atrophy in Fischer-344 rats." in: Biogerontology, Vol. 15, Issue 5, pp. 517-534, (2015) (PubMed).

  • Target
    NRF1 (Nuclear Respiratory Factor 1 (NRF1))
    Alternative Name
    Nrf1 (NRF1 Products)
    Background
    Background: NRF1 (also known as nuclear respiratory factor 1, alpha palindromic binding protein and alpha-pal) is the mammalian homolog to the erect wing (ewg) Drosophila protein that is required for proper development of the central nervous system and indirect flight muscles. In mammals NRF1 functions as a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. This protein links the transcriptional modulation of key metabolic genes to cellular growth and development and has been implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.  NRF1 forms a homodimer and binds DNA as a dimer.  NRF1 shows a nuclear localization and is widely expressed in embryonic, fetal, and adult tissues.  Phosphorylation of NRF1 enhances DNA binding.  Multiple splice variants have been identified for this protein.
    Gene ID
    18181, 13529317
    UniProt
    Q9WU00
    Pathways
    Regulation of Lipid Metabolism by PPARalpha
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