NRF1 antibody (AA 1-534)

Catalog No. ABIN129526

Supplier Product No.: 200-401-869

This Rabbit Polyclonal antibody specifically detects NRF1 in WB, ELISA and IHC. It exhibits reactivity toward Human and Mouse and has been mentioned in 3+ publications.

Quick Overview for NRF1 antibody (AA 1-534) (ABIN129526)

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NRF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-NRF1 Antibody

Binding Specificity: AA 1-534

Supplier Product No.: 200-401-869

Supplier: Rockland

Purpose: NRF1 Antibody

Cross-Reactivity (Details): This protein A purified antibody is directed against mouse NRF1.

Characteristics: Synonyms: rabbit anti-NRF1 antibody, rabbit anti-NRF 1 antibody, NRF-1, alpha pal antibody, alpha palindromic binding protein antibody, locus control region factor 1 antibody, NFE2 related factor 1 antibody, nuclear respiratory factor 1 antibody, transcription factor 11 antibody

Purification: The product was purified from monospecific antiserum by protein A affinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant mouse NRF1 protein corresponding to aa 1- 534 of the native protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 2 mg/mL - 5 μg/mL

Application Note: This protein A purified antibody has been tested for use in ELISA, immunohistochemistry and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 67 kDa in size corresponding to NRF1 by western blotting in the appropriate cell lysate or extract.  Splice variants exist for this protein that may result in the detection of lower molecular weight bands.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:3,000 - 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.3 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-NRF1 antibody (ABIN129526)

Hinkley, Morton, Ichinoseki-Sekine, Huertas, Smuder: "Exercise Training Prevents Doxorubicin-induced Mitochondrial Dysfunction of the Liver." in: Medicine and science in sports and exercise, Vol. 51, Issue 6, pp. 1106-1115, (2019) (PubMed).

Miura, Saitoh, Kokubun, Owada, Yamauchi, Machii, Takeishi: "Mitochondrial-Targeted Antioxidant Maintains Blood Flow, Mitochondrial Function, and Redox Balance in Old Mice Following Prolonged Limb Ischemia." in: International journal of molecular sciences, Vol. 18, Issue 9, (2018) (PubMed).

Joseph, Nguyen, Welter, Dominguez, Behnke, Adhihetty: "Mitochondrial adaptations evoked with exercise are associated with a reduction in age-induced testicular atrophy in Fischer-344 rats." in: Biogerontology, Vol. 15, Issue 5, pp. 517-534, (2015) (PubMed).

Target Details for NRF1

Target: NRF1 (Nuclear Respiratory Factor 1 (NRF1))

Alternative Name: Nrf1

Background: Background: NRF1 (also known as nuclear respiratory factor 1, alpha palindromic binding protein and alpha-pal) is the mammalian homolog to the erect wing (ewg) Drosophila protein that is required for proper development of the central nervous system and indirect flight muscles. In mammals NRF1 functions as a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. This protein links the transcriptional modulation of key metabolic genes to cellular growth and development and has been implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.  NRF1 forms a homodimer and binds DNA as a dimer.  NRF1 shows a nuclear localization and is widely expressed in embryonic, fetal, and adult tissues.  Phosphorylation of NRF1 enhances DNA binding.  Multiple splice variants have been identified for this protein.

Gene ID: 18181, 13529317

UniProt: Q9WU00

Pathways: Regulation of Lipid Metabolism by PPARalpha