MAD1L1 antibody

Catalog No. ABIN129517

Supplier Product No.: 200-301-903

Product Details anti-MAD1L1 Antibody

Target: MAD1L1 (MAD1 Mitotic Arrest Deficient-Like 1 (MAD1L1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAD1L1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Supplier Product No.: 200-301-903

Supplier: Rockland

Purpose: MAD1L1 Antibody

Cross-Reactivity (Details): This Protein A purified antibody is directed against human MAD1L1 protein.

Characteristics: Synonyms: mouse anti-MAD1L1 antibody, Mitotic arrest deficient 1 antibody, Mitotic checkpoint MAD1 protein homolog antibody, Mitotic spindle assembly checkpoint protein MAD1 antibody, PIG9 antibody, Tax binding protein 181 antibody

Purification: The product was purified from tissue culture supernatant by chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with full-length recombinant human MAD1L1 protein.

Immunogen Type: Recombinant Protein

Clone: 9B10

Isotype: IgG2b kappa

Application Details

Application Notes:

Application Note: This protein A purified antibody is suitable for use in flow cytometry, immunoprecipitation, immunofluorescence and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 83 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:200 - 1:1,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-MAD1L1 antibody (ABIN129517)

Sikorski, Mehta, Inngjerdingen, Thakor, Kling, Kalina, Nyman, Stensland, Zhou, de Souza, Holden, Stuchly, Templin, Lund-Johansen: "A high-throughput pipeline for validation of antibodies." in: Nature methods, Vol. 15, Issue 11, pp. 909-912, (2019) (PubMed).

Target Details for MAD1L1

Target: MAD1L1 (MAD1 Mitotic Arrest Deficient-Like 1 (MAD1L1))

Alternative Name: MAD1L1 (MAD1L1 Products)

Background: Background: MAD1L1 (also called mitotic spindle assembly checkpoint protein, MAD1A, MAD1-like 1 and HsMAD1) is a component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD1L1 has a role in the correct positioning of the septum and is required for anchoring MAD2L1 to the nuclear periphery.   MAD1L1 forms a homodimer and also heterodimerizes with MAD2L1 in order to form a tetrameric MAD1L1-MAD2L1 core complex. Perturbation of the original MAD1L1-MAD2L1 structure by the spindle checkpoint may decrease MAD2L1 affinity for MAD1L1. CDC20 can compete with MAD1L1 for MAD2L1 binding, until the attachment and/or tension dampen the checkpoint signal, preventing further release of MAD2L1 on to CDC20.  MAD1L1 is also able to interact with the BUB1/BUB3 complex and the viral Tax protein.   MAD1L1 is a nuclear protein that is seen to move from the beginning to the end of mitosis from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody.  Multiple isoforms may exist for this protein (MAD1L1 and MAD1L2).  MAD1L1 is induced by TP53 and is phosphorylated by BUB1. MAD1L1 is hyperphosphorylated in late S through M phases or after mitotic spindle damage.  Defects in MAD1L1 are involved in the development and/or progression of various types of cancer.

Gene ID: 8379

UniProt: Q9Y6D9

Pathways: M Phase