CENPE antibody

Catalog No. ABIN129515

Supplier Product No.: 200-301-901

Product Details anti-CENPE Antibody

Target: CENPE (Centromere Protein E, 312kDa (CENPE))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CENPE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Supplier Product No.: 200-301-901

Supplier: Rockland

Purpose: CENP-E

Cross-Reactivity (Details): This Protein A purified antibody is directed against human CENP-E protein.

Characteristics: Synonyms: mouse anti-CENP-E antibody, Centromere-associated protein E, Centromeric protein E antibody, KIF10 antibody, Kinesin-7, Kinesin family member 10 antibody, Kinesin-related protein CENPE

Purification: The product was purified from tissue culture supernatant by chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with a full length recombinant protein corresponding to human CENP-E protein.

Immunogen Type: Recombinant Protein

Clone: 1H12

Isotype: IgG1

Application Details

Application Notes:

Application Note: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein.  Specific conditions for reactivity should be optimized by the end user.  Expect a predominant band at ~ 320 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: 1:200

ELISA Dilution: 1:5,000 - 1:20,000

IF Microscopy Dilution: 1:500 - 1:2,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CENPE

Target: CENPE (Centromere Protein E, 312kDa (CENPE))

Alternative Name: CENPE (CENPE Products)

Background: Background: CENP-E, Centrosome-associated protein E, is a kinesin-like minus-end directed microtubule motor protein that accumulates in the G₂ phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENP-E is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation. CENP-E interacts with CENP-F and BUBR1 kinase.  CENP-E associates with kinetochores during congression, relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division.

Gene ID: 1062

UniProt: Q02224

Pathways: M Phase, Maintenance of Protein Location