CENPE antibody

Catalog No. ABIN129515

Product Details anti-CENPE Antibody

Target: CENPE (Centromere Protein E, 312kDa (CENPE))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CENPE antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with a full length recombinant protein corresponding to human CENP-E protein.
Immunogentype:Recombinant

Clone: 1H12

Isotype: IgG

Application Details

Application Notes: This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein.  Specific conditions for reactivity should be optimized by the end user.  Expect a predominant band at ~ 320 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for CENPE

Target: CENPE (Centromere Protein E, 312kDa (CENPE))

Alternative Name: CENP-E (CENPE Products)

Synonyms: CENP-E antibody, KIF10 antibody, PPP1R61 antibody, 312kDa antibody, AU019344 antibody, BC049989 antibody, C530022J18 antibody, Kif10 antibody, CENPE antibody, kif10 antibody, cenp-E antibody, XCENP-E antibody, wu:fc06a10 antibody, zgc:152925 antibody, centromere protein E antibody, centromere protein E, 312kDa antibody, CENPE antibody, Cenpe antibody, cenpe antibody

Background: CENP-E, Centrosome-associated protein E, is a kinesin-like minus-end directed microtubule motor protein that accumulates in the G₂ phase of the cell cycle. Unlike other centrosome-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometa-phase. CENP-E is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation. CENP-E interacts with CENP-F and BUBR1 kinase.  CENP-E associates with kinetochores during congression, relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division.
Synonyms: Centromeric protein E antibody, KIF10 antibody, Kinesin family member 10 antibod

Gene ID: 1062

UniProt: Q02224

Pathways: M Phase, Maintenance of Protein Location