PMS2 antibody
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- Target See all PMS2 Antibodies
- PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This PMS2 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA
- Supplier Product No.
- 200-301-864
- Supplier
- Rockland
- Purpose
- PMS2 Antibody
- Cross-Reactivity (Details)
- The epitope was putatively mapped to amino acids 58-81 of human PMS2.
- Characteristics
- Synonyms: mouse anti-PMS2 antibody, DNA mismatch repair gene homologue antibody, H_DJ0042M02.9 antibody, HNPCC4 antibody, PMS 2 antibody, PMS1 protein homolog 2 antibody, PMS2 postmeiotic segregation increased 2 antibody, PMS2CL antibody, PMSL2 antibody
- Purification
- This is an protein A purified antibody from ascites fluid directed against PMS2-134 and reacts with full length version of PMS2 in human and hamster tissues.
- Sterility
- Sterile filtered
- Immunogen
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Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human PMS2 corresponding to the first 133 amino acid residues of the protein. The clone was produced using conventional hybridoma technology.
Immunogen Type: Recombinant Protein
- Clone
- 1204
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product PMS2 Primary Antibody
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- Application Notes
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Application Note: Anti-PMS2 antibody has been tested for use in ELISA and western blotting. This product is suitable for immunoprecipitation. Specific conditions for western blotting reactivity should be optimized by the end user. Expect a band approximately 96 kDa in size corresponding to human PMS2 by western blotting in most cell lines and tissues as PMS2 is ubiquitously expressed.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:5,000 - 1:20,000
Other: User Optimized
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.38 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae) (PMS2))
- Alternative Name
- PMS2 (PMS2 Products)
- Background
- Background: PMS2 is a highly conserved nuclear protein involved in mismatch repair during DNA replication and has been identified to be composed as a heterodimer of PMS2 and MLH1. PMS is part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Defects in PMS2 are the cause of hereditary non-polyposis colorectal cancer type 4 (HNPCC4), Turcot syndrome (an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas) and supratentorial primitive neuroectodermal tumors with cafe-au-lait spots (SNTCL). The human PMS2 gene encodes an 862 aa, 96 kDa polypeptide.
- Gene ID
- 5395
- UniProt
- P54278
- Pathways
- DNA Damage Repair, Production of Molecular Mediator of Immune Response
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