MAD2L1 antibody

Catalog No. ABIN129510

Product Details anti-MAD2L1 Antibody

Target: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAD2L1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Purpose: MAD2L1 Antibody

Cross-Reactivity (Details): This Protein A purified antibody is directed against human MAD2L1 protein.

Characteristics: Synonyms: mouse anti-MAD2L1 antibody, Mitotic arrest deficient yeast homolog like 1 antibody, Mitotic spindle assembly checkpoint protein antibody

Purification: The product was purified from tissue culture supernatant by chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with full-length recombinant human MAD2L1 protein.

Immunogen Type: Recombinant Protein

Clone: 17D10

Isotype: IgG1

Application Details

Application Notes:

Application Note: This protein A purified antibody has been tested for use in immunoprecipitation and by western blot.  This antibody is not applicable for immunofluorescence microscopy.  Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 24 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:200 - 1:2,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MAD2L1

Target: MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))

Alternative Name: MAD2L1 (MAD2L1 Products)

Background: Background: MAD2L1 (also called mitotic spindle assembly checkpoint protein, MAD2A, MAD2-like 1 and HsMAD2) is a component of the mitotic spindle assembly checkpoint monitors the process of kinetochore-spindle attachment and delays the onset of anaphase when this process is not complete. MAD2L1 inhibits the activity of the anaphase-promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. This protein has a nuclear localization.  A MAD2 pseudogene has been mapped to chromosome 14.

Gene ID: 4085, 4505067

UniProt: Q13257