Keratin 6C antibody

Catalog No. ABIN129508

Supplier Product No.: 200-301-390

Product Details anti-Keratin 6C Antibody

Target: Keratin 6C (KRT6C)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Keratin 6C antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunoprecipitation (IP), Fluorescence Microscopy (FM), Immunofluorescence (IF), Multiplex Assay (MA)

Supplier Product No.: 200-301-390

Supplier: Rockland

Purpose: Keratin Antibody

Cross-Reactivity (Details): This antibody reacts with keratin (56 kDa), keratin 17 (46 kDa), keratin 18 (45 kDa) and keratin 19 (40 kDa) derived from humans.

Characteristics: Synonyms: mouse anti-keratin antibody, mouse anti-cytokeratin 6 antibody, PC2 antibody, Type II keratin isoform K6c antibody

Purification: This protein A purified mouse monoclonal antibody reacts specifically with keratins from human tissues and derived cell lines.

Sterility: Sterile filtered

Immunogen:

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with purified human cytoskeletal preparations from A431 cells.

Immunogen Type: Native Protein

Clone: C11

Isotype: IgG1

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:50 - 1:200

Application Note: Anti-Keratin Antibody has been tested in ELISA, immunohistochemistry, immunofluorescence, immunoblotting and immunoprecipitation.  For a positive control use skin, colon carcinoma and squamous granulocyte carcinoma cells.

Western Blot Dilution: 1:50 - 1:200

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

IF Microscopy Dilution: 1:50 - 1:200

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Keratin 6C

Target: Keratin 6C (KRT6C)

Alternative Name: KRT6C (KRT6C Products)

Background: Background: Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. There are two types of keratins (cytoskeletal and microfibrillar) and are clustered in a region of chromosome. Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (i.e. skin) and non-keratinizing cells (i.e. mesothelial). Anti-Keratin Antibody is useful for researchers interested in cytoskeletal signaling and developmental biology research.

Gene ID: 286887

UniProt: P48668