Insulin antibody

Catalog No. ABIN129507

Supplier Product No.: 200-301-389

Product Details anti-Insulin Antibody

Target: Insulin (INS)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Insulin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Immunoprecipitation (IP), Dot Blot (DB)

Supplier Product No.: 200-301-389

Supplier: Rockland

Purpose: Insulin Antibody

Cross-Reactivity (Details): This protein A purified mouse monoclonal antibody reacts specifically with insulin from human and swine sources. Cross reactivity with insulin from mouse and rat does not occur.

Characteristics: Synonyms: mouse anti-insulin antibody, ILPR antibody, INS antibody, Insulin A chain antibody, Insulin B chain antibody, Insulin precursor antibody, IRDN antibody, Proinsulin antibody, Proinsulin precursor antibody

Purification: Protein A purified

Sterility: Sterile filtered

Immunogen:

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with purified human insulin coupled to bovine serum albumin (BSA).

Immunogen Type: Native Protein

Clone: 2D11-H5

Isotype: IgG1 kappa

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:50 - 1:200

Application Note: Mouse Anti-Insulin Antibody has been tested by ELISA and dot blot and is suitable for immunohistochemistry, immunoblotting and immunoprecipitation. Insulin has a cytoplasmic localization.  Pancreatic tissue or b-cells from islets of Langerhans can be used as a positive control.

Western Blot Dilution: 1:50 - 1:200

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Insulin

Target: Insulin (INS)

Alternative Name: INS (INS Products)

Background: Background: Recognizes the 51 amino acid (6 kDa) insulin polypeptide composed of A and B chains. Proinsulin, which has very little biological activity, is cleaved by proteases within its cell of origin into the insulin molecule and the C-peptide basic residue. Insulin enhances membrane transport of glucose, amino acids, and certain acids. It also promotes glycogen storage, formation of triglycerides, and synthesis of proteins and nucleic acids.  The main storage site for insulin is the pancreatic islets.  Antibodies to insulin are important as b-cell and tumor (insulinoma) markers.

Gene ID: 3630

UniProt: P01308

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation