Histone H4 antibody (N-Term)

Catalog No. ABIN127256

Product Details anti-Histone H4 Antibody

Target: Histone H4

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H4 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The antibody detects ~11 kDa Histone H4 protein. It does not cross-react with other Histones.

Purification: Affinity Chromatography.

Immunogen: Synthetic peptide corresponding to the N-terminus of Human Histone H4.

Application Details

Application Notes: Western Blot: 0.5-4.0 μg/mL. Immunoprecipitation: 20-40 μg/mL. Immunohistochemistry: 20-40 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2 containing 0.01 % Thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for Histone H4

Target: Histone H4

Abstract: Histone H4 Products

Background: The nucleosome is made up of four core histone proteins (H2A, H2B, H3 and H4) and is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranscriptional modification including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression.Synonyms: H4/A, H4FA, HIST1H4

Gene ID: 121504

NCBI Accession: NP_001029249

UniProt: P62805