Cathepsin B antibody
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- Target See all Cathepsin B (CTSB) Antibodies
- Cathepsin B (CTSB)
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Cathepsin B antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- The purified antibody detects both the proform (37-43 kDa) and the mature form (25 kDa) of Cathepsin B.
- Cross-Reactivity (Details)
- Species reactivity (tested):Human, Mouse and Rat.
- Purification
- Affinity Chromatography.
- Immunogen
- Synthetic peptide surrounding amino acid 322 of human cathepsins B
- Top Product
- Discover our top product CTSB Primary Antibody
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- Application Notes
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Western Blot: 0.5-4 μg/mL. Immunohistochemistry: 10-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- PBS, pH 7.2 containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal.
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C/-80 °C
- Storage Comment
- Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -20 °C to -70 °C.
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- Target
- Cathepsin B (CTSB)
- Alternative Name
- Cathepsin B (CTSB Products)
- Synonyms
- APPS antibody, CPSB antibody, CB antibody, apps antibody, cpsb antibody, LOC692390 antibody, CTSB antibody, cathepsin B antibody, cathepsin B S homeolog antibody, CTSB antibody, Ctsb antibody, ctsb.S antibody, LOC692390 antibody
- Background
- Cathepsin B is a normal lysosomal cysteine proteinase that is expressed in all cells. It is synthesized as an inactive 43 kDa pro-enzyme which, by removal of a 62 amino acid propeptide, is activated to the single-chain form of 31 kDa or the two-chain form (25 and 5 kDa subunits). Matured cathepsin B is normally localized in the lysosomes where it functions in protein turnover. Cathepsin B is also shown capable of degrading extracellular matrix proteins. Elevated levels of cathepsin B correlate with malignancy suggesting that this enzyme may be a useful prognostic marker for several types of human cancers.Synonyms: APP secretase, APPS, CPSB, CTSB, Cathepsin B1
- Gene ID
- 1508
- NCBI Accession
- NP_001899
- UniProt
- P07858
- Pathways
- Activation of Innate immune Response, Toll-Like Receptors Cascades
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