His Tag antibody
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- Target See all His Tag Antibodies
- His Tag
- Reactivity
- Please inquire
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This His Tag antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
- Sequence
- HHHHHH
- Characteristics
- Synonyms: 6xHis-Tag, His Tag, HIS6 Tag, HHHHHH Tag
- Clone
- BV-G020
- Isotype
- IgG2b
- Top Product
- Discover our top product His Tag Primary Antibody
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- Application Notes
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Western Blot: 1-2 μg/mL. Immunoprecipitation: 10-20 μg/mL. Immunocytochemistry: 10-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.5 mg/mL
- Buffer
- PBS, pH 7.2 containing 50 % Glycerol, 1 % BSA as stabilizer and 0.02 % Thimerosal as preservative.
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C/-80 °C
- Storage Comment
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Store the antibody undiluted at -20 °C or (in aliquots) at -70 °C for long term storage . Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - Expiry Date
- 12 months
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- Target
- His Tag
- Alternative Name
- 6xHistidine Epitope Tag (His Tag Products)
- Target Type
- Tag
- Background
- The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification.
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