NF-kB p65 antibody
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- Target See all NF-kB p65 (NFkBP65) Antibodies
- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
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Reactivity
- Human, Mouse, Rat
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This NF-kB p65 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Specificity
- This antibody recognizes NFkB.
- Cross-Reactivity (Details)
- Species reactivity (tested):Human, Mouse and Rat.
- Characteristics
- Synonyms: NF kappa B p65, NFkB p65, Transcription factor p65, Rel A, NFKB3, Nuclear factorNF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer inB-cells 3
- Purification
- Affinity Chromatography.
- Immunogen
- Synthetic peptide from Human NFkB. Remarks: Molecular Weight: 65 kDa
- Clone
- S8 (2A12A7)
- Isotype
- IgG1
- Top Product
- Discover our top product NFkBP65 Primary Antibody
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- Application Notes
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Western Blot: 1-4 μg/mL. Immunoprecipitation. Immunohistochemistry. A431 cells can be used as a Positive Control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- PBS containing 1 mg/mL BSA, 1.5 mM Sodium Azide and 50 % Glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C/-80 °C
- Storage Comment
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Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - Expiry Date
- 12 months
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- Target
- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
- Alternative Name
- RELA / NF-kB p65 (NFkBP65 Products)
- Synonyms
- C-Rel antibody, NFKB3 antibody, p65 antibody, NFkB antibody, c-rel antibody, zgc:100833 antibody, Xrel2 antibody, Xrel3 antibody, rel antibody, rel-A antibody, rel2 antibody, rel3 antibody, v-rel antibody, xrel antibody, REL proto-oncogene, NF-kB subunit antibody, RELA proto-oncogene, NF-kB subunit antibody, v-rel reticuloendotheliosis viral oncogene homolog A (avian) antibody, v-rel avian reticuloendotheliosis viral oncogene homolog antibody, v-rel avian reticuloendotheliosis viral oncogene homolog L homeolog antibody, REL antibody, RELA antibody, Rela antibody, Rel antibody, rel antibody, rel.L antibody
- Background
- NFkB was identified as a sequence specific transcriptional activator that binds to the intronic enhancer of kappa light chain gene in B lymphocytes. NFkB is a heterodimer that consists of a 50 kDa DNA binding subunit (p50) and a 65 kDa transactivation subunit (p65/RelA). Both of these subunits exhibit sequence homology to the protooncogene c-Rel. The p50/p65 heterodimer remains in the cytosol in an inactive form as a complex with its inhibitor, IkB. Upon stimulation of cells by a wide variety of stimuli such as lipopolysaccharide (LPS), proinflammatory cytokines, and viral infection. IkB is phosphorylated and degraded by proteosome. The active NFkB heterodimer is translocated into the nucleus and induces gene expression.Synonyms: NF kappa B p65, NFKB3, NFkB p65, Nuclear factor NF-kappa-B p65 subunit, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, Rel A, Transcription factor p65
- Gene ID
- 5970
- UniProt
- Q04206
- Pathways
- NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
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