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Vimentin antibody

VIM Reactivity: Human, Mouse, Dog WB Host: Mouse Monoclonal 11H6 unconjugated
Catalog No. ABIN126914
  • Target See all Vimentin (VIM) Antibodies
    Vimentin (VIM)
    Reactivity
    • 304
    • 120
    • 116
    • 60
    • 60
    • 49
    • 42
    • 36
    • 29
    • 21
    • 20
    • 13
    • 13
    • 8
    • 8
    • 6
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Dog
    Host
    • 158
    • 129
    • 11
    • 6
    • 6
    • 5
    • 2
    Mouse
    Clonality
    • 172
    • 144
    Monoclonal
    Conjugate
    • 197
    • 15
    • 13
    • 12
    • 9
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    This Vimentin antibody is un-conjugated
    Application
    • 262
    • 115
    • 104
    • 97
    • 88
    • 64
    • 48
    • 40
    • 39
    • 33
    • 23
    • 20
    • 8
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Specificity
    This antibody specifically recognizes Vimentin at 54 kDa in Western-blot applications.
    Purification
    Size exclusion chromatography
    Components
    incl. positive Control
    Immunogen
    Synthetic peptide conjugated to hemocyanin
    Clone
    11H6
    Isotype
    IgG
    Top Product
    Discover our top product VIM Primary Antibody
  • Application Notes
    Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 1 mL H2O (15 min, RT).
    Buffer
    1 mL2 x PBS containing 0.09 % Sodium Azide/PEG and Sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C/-80 °C
    Storage Comment
    Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
    Shelf life: one year from despatch.
    Expiry Date
    12 months
  • Target
    Vimentin (VIM)
    Alternative Name
    Vimentin (VIM Products)
    Background
    Vimentin is a major subunit protein of intermediate filaments found in mesenchymal cells. Vimentin is expressed in many tumors/tumor cell lines after epithelial to mesenchymal transition.Synonyms: VIM
    Molecular Weight
    54 kDa
    Gene ID
    7431
    UniProt
    P08670
    Pathways
    Caspase Cascade in Apoptosis
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