Phosphotyrosine antibody (phosphorylated)

Catalog No. ABIN126874

Product Details anti-Phosphotyrosine Antibody

Target: Phosphotyrosine

Binding Specificity: phosphorylated

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phosphotyrosine antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Purification: Size exclusion chromatography

Components: incl. positive Control

Immunogen: Synthetic phosphopeptide conjugated to KLH

Clone: 2C8

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 basedblocking and blot incubation buffer. ELISA: 0.1 μg/mL. Immunoprecipitation: 1 - 10 g per 106 pervanadate-treated A431 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Phosphotyrosine MW standardSize: 20 BlotsFormulation: Phosphotyrosine modified standard proteins lyophilized from PBS / 0. 1 %SDS / PEG / Sucrose / Malachitgreen. The following standard proteins were modified with phosphotyrosine: galactosidase (116kD), phosphorylase A (98 kD), BSA (67 kD), ovalbumin (46 kD), carbonic anhydrase (32kD), and soybean trypsin inhibitor (24 kD). Storage: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The phosphptyrosine molecular weight marker is recommended forimmunoblot applications. Use 20 µl of the phosphotyrosine molecular weight marker perlane (mini gel). The individual proteins of the marker are recognized by the following commerciallyavailable clones: 2C8, 1F9, 3B12, 9F1, 9H8, 16F4.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -20 °C to -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Phosphotyrosine

Target: Phosphotyrosine

Abstract: Phosphotyrosine Products

Target Type: Amino Acid

Background: Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosine kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via interaction of phosphotyrosine residues with SH2 or PID domains.