Phosphothreonine antibody (phosphorylated)

Catalog No. ABIN126869

Product Details anti-Phosphothreonine Antibody

Target: Phosphothreonine

Binding Specificity: phosphorylated

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phosphothreonine antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Rat and Dog.

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Components: incl. positive Control

Immunogen: Synthetic phosphopeptide conjugated to KLH

Clone: 14B3

Isotype: IgG1

Application Details

Application Notes: ELISA: 0.05 μg/mL. Immunoprecipitation: 1-10 μg per 10^6 pervanadate-treated A431 cells. Western Blot (Immunoblotting): 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: BSA/Tween 20 based blocking buffer. DO NOT USE MILKOR CASEIN FOR BLOCKING!
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Positive Control: pSer / pThr Molecular Weight MarkerFormulation:

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °Cand reconstituted (aliquote and freeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 1 week.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Phosphothreonine

Target: Phosphothreonine

Abstract: Phosphothreonine Products

Target Type: Amino Acid

Background: Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein-interactions. Modification of proteins on serine residues is mediated by serine/threonine kinases.