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MBP Tag antibody

WB, EIA Host: Mouse Monoclonal 6-00E-004 unconjugated
Catalog No. ABIN126834
  • Target
    MBP Tag
    Host
    • 12
    • 5
    Mouse
    Clonality
    • 11
    • 5
    Monoclonal
    Conjugate
    • 15
    • 1
    • 1
    Un-conjugated
    Application
    • 15
    • 8
    • 7
    • 5
    • 3
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Specificity
    This antibody can be used for the detection of MBP fusion proteins.
    Characteristics
    Synonyms: MMBP, Maltodextrin-binding protein, malE-Tag
    Purification
    Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
    Immunogen
    MBP (Maltose Binding Protein)
    Clone
    6-00E-004
    Isotype
    IgG1
  • Application Notes
    Western blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 1 mL H2O (15 min, RT)
    Buffer
    1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C/-80 °C
    Storage Comment
    Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
    Shelf life: one year from despatch.
    Expiry Date
    12 months
  • Target
    MBP Tag
    Alternative Name
    Maltose Binding Protein Tag / MBP-Tag
    Target Type
    Tag
    Background
    Maltose Binding Protein (MBP), the E. coli malE gene product, is widely used as fusion partner for the expression of recombinant proteins.Synonyms: MMBP, Maltodextrin-binding protein, malE-Tag
    Molecular Weight
    43 kDa
    Gene ID
    948538
    UniProt
    P0AEX9
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