Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Immunohistochemistry on frozen sections. Positive Control: Cell lysate from untreated A431 cells. (See Protocol) Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol
Positive Control Cell Lysate (A431 Untreated)
Restrictions
For Research Use only
Reconstitution
Restore with 1 mL H2O (15 min, RT).
Buffer
1 mLPBS containing 0.09 % Sosium Azide, PEG and Sucrose
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C/-80 °C
Storage Comment
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Shelf life: One year from despatch.
Expiry Date
12 months
Target
JNK2 (MAPK9)
(Mitogen-Activated Protein Kinase 9 (MAPK9))
Stress-activated Protein Kinases (SAPKs) are strongly activated in response to adverse stimuli such as heat and osmotic shock, UV light and other DNA-damaging reagents, and inhibitors of protein synthesis. They are also activated strongly in response to agonists that are released or produced under conditions of stress, such as proinflammatory cytokines. The SAPK1/jnk family consists of 3 isoforms: SAPK1α/jnk2, SAPK1β/jnk3, SAPK1γ/jnk1.Synonyms: JNK-55, MAP kinase 9, Mitogen-activated protein kinase 9, PRKM9, Stress-activated protein kinase JNK2, c-Jun N-terminal kinase 2