Synaptophysin antibody

Catalog No. ABIN126707

Product Details anti-Synaptophysin Antibody

Target: Synaptophysin (SYP)

Reactivity: Human, Mouse, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Synaptophysin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: SY38 represents an excellent Marker for several neuroendocrine, neuronal and adrenal tumors. Neuronal and adrenal tumors such as pheochromocytomas, paragangliomas, neuroblastomas, ganglioneuroblastomas. Neuroendocrine tumors of epithelial origin: Pancreatic islet cell carcinoma, bronchial and gastrointestinal carcinoids, medullary carcinoma of thyroid. Polypeptide Reacting: Mr 38 000 transmembrane glycoprotein of presynaptic vesicles. Reactivity on Cultured Cell Lines: Rat PC-12 cell line.

Purification: Affinity Chromatography on Protein A.

Immunogen: Synaptophysin from presynaptic vesicles, prepared from bovine brain.

Clone: SY38

Isotype: IgG1

Application Details

Application Notes: Immunoblotting. Immunofluorescence. Cytological Material. Immunohistochemistry on Frozen and Paraffin Sections: 1/50 with PBS, pH 7.4. In no case Protease Pretreatment. Incubation Time: 1 h at RT, extended with Paraffin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 1 mL distilled water.

Buffer: Final Solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody (in aliquots) at -20 °C.

Target Details for Synaptophysin

Target: Synaptophysin (SYP)

Alternative Name: Synaptophysin / SYP (SYP Products)

Background: Synaptophysin is a glycoprotein present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by neuroendocrine cells throughout the body, both normal and neoplastic. Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms. This antibody reacts with neuroendocrine cells of human adrenal medulla, carotid body, skin, pituitary gland, thyroid, lung, pancreas, and gastointestinal mucosa. It also reacts with a wide spectrum of neuroendocrine neoplasms of neural type including neuroblastomas, ganglioneuroblastomas, ganglioneuromas, pheochromocytomas, chromaffin, and non chromaffin paragangliomas.Synonyms: Major synaptic vesicle protein p38, Neuroendocrine Marker

Gene ID: 6855

NCBI Accession: NP_003170

UniProt: P08247

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity