Keratin Basic antibody

Catalog No. ABIN125988

Product Details

Target: Keratin Basic

Reactivity: Human, Rat, Cow, Amphibian

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody represents an excellent marker for distinguishing carcinomas from non-epithelial tumors. Polypeptides Reacting: Mr 52 500-Mr 68 000 keratins (type II keratins K1-K8, formerly also designated Cytokeratins 1-8) of Human epithelial cells. Tumors Specifically Detected: All epithelium-derived neoplasms. Tested Reactivities on Cultured Cell Lines: MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650, SSC-12, Bovine BMGE+H, BMGE-H, MDBK.

Cross-Reactivity (Details): Species reactivity (tested):Human, Bovine, Rat, Amphibia.

Purification: Affinity Chromatography on Protein A

Immunogen: Cytoskeletal proteins from cultured Human MCF-7 cells

Clone: Ks pan 1-8

Isotype: IgG2a

Application Details

Application Notes: Immunoblotting. Immunofluorescence. Immunohistochemistry on frozen and cytological material: 1: 10, 1h at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 1 mL distilled water

Buffer: Final solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Target Details

Target: Keratin Basic

Abstract: Keratin Basic Products

Background: Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1 (67), CK2 (65.5), CK3 (64), CK4 (59), CK5 (58), CK6 (56), CK7 (54), CK8 (52.5) and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10 (56.5), CK12 (56), CK13 (53), CK14 (50), CK16( 48), CK17 (46), CK18 (45), CK19(48) and CK20(46).