M13 Bacteriophage G8P Capsid antibody

Catalog No. ABIN125967

Product Details

Target: M13 Bacteriophage G8P Capsid

Reactivity: Bacteriophage M13

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Immunoelectron Microscopy (IEM)

Specificity: B62-FE2 binds to an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT (cf. Kneissel et al.).

Purification: Affinity Chromatography on Protein A

Immunogen: fd phages from E. coli F+ strain (JM109)

Clone: B62-FE2

Isotype: IgG2b

Application Details

Application Notes: Phage Display: immunoassay for identification of recombinant antigen- orantibody-phages, Detection limit: 10e7 phage particles. Immunoblotting. Immunoelectron microscopy. ELISA: 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in 1.0 mL distilled water.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Target Details

Target: M13 Bacteriophage G8P Capsid

Target Type: Phage Protein

Background: Synonyms: Coat protein B, Gene 8 protein, Major coat protein

Gene ID: 927333

NCBI Accession: NP_510890

UniProt: P69541