Hsp90C antibody (C-Term)

Catalog No. ABIN125892

Product Details anti-Hsp90C Antibody

Target: Hsp90C (Alfa-Hsp90C, Heat Shock Protein (Hsp90C))

Binding Specificity: C-Term

Reactivity: Chlamydomonas reinhardtii

Host: Rabbit

Clonality: Polyclonal

Application: Immunoprecipitation (IP), Western Blotting (WB)

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: Ostreococcus sp.

Characteristics: Expected / apparent Molecular Weight of the Antigene: 89 kDa

Purification: serum

Immunogen: C-terminal 238 amino acids of HSP90C, Q66T67, expressed with N- and C-terminal hexahistidine tags in E. coli, purified with Ni-NTA

Application Details

Application Notes: 1 : 3000 with standard ECL (WB)

Comment:

HSP90C protein is easily degraded and degradation products are detected by this antibody.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References for anti-Hsp90C antibody (ABIN125892)

Willmund, Schroda: "HEAT SHOCK PROTEIN 90C is a bona fide Hsp90 that interacts with plastidic HSP70B in Chlamydomonas reinhardtii." in: Plant physiology, Vol. 138, Issue 4, pp. 2310-22, (2005) (PubMed).

Target Details for Hsp90C

Target: Hsp90C (Alfa-Hsp90C, Heat Shock Protein (Hsp90C))

Background: HSP90C is a nuclear-encoded, chloroplast-targeted chaperone of the HSP90 family. It forms dimers that interact with HSP70B. HSP90C displays a weak ATPase activity that is inhibited by radicicol.

Molecular Weight: 89 kDa

UniProt: Q66T67