Hcf101 antibody (AA 206-532)

Catalog No. ABIN125887

Product Details anti-Hcf101 Antibody

Target: Hcf101 (High-Chlorophyll-Fluorescence 101 (Hcf101))

Binding Specificity: AA 206-532

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Application: Immunolocalization (IL), Western Blotting (WB)

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: Arabidopsis thaliana

Characteristics: Expected / apparent Molecular Weight of the Antigene: 57 / 50.5 kDa

Purification: serum

Immunogen: recombinant Arabidopsis thaliana Hcf101, amino acid residues 206-532 Q6STH5, At3g24430

Application Details

Application Notes: 1:3000 with standard ECL (WB), 1: 3000 (IL)

Comment:

Immunolocalization has been done on isolated plastids, followed by separation into stroma and thylakoids

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

References for anti-Hcf101 antibody (ABIN125887)

Shang, Yan, Liu, Cao, Mei, Xin, Wu, Wang, Du, Jiang, Zhang, Zhao, Sun, Liu, Yu, Zhang: "The Mg-Chelatase H Subunit of Arabidopsis Antagonizes a Group of Transcription Repressors to Relieve ABA-Responsive Genes of Inhibition." in: The Plant cell, (2010) (PubMed).

Target Details for Hcf101

Target: Hcf101 (High-Chlorophyll-Fluorescence 101 (Hcf101))

Alternative Name: High chlorophyll fluorescence phenotype protein (Hcf101)

Synonyms: HIGH-CHLOROPHYLL-FLUORESCENCE 101 antibody, ATP binding protein antibody, fe-S cluster assembly factor HCF101, chloroplastic antibody, HCF101 antibody, LOC9321711 antibody

Background: AGI Code: At3g24430
Hcf101 is a novel protein required for photosystem I biogenesis. Hcf101-1 is a high-chlorophyll-fluorescence hcf) Arabidopsis thaliana mutant that lacks photosystem I. In green tissue the level of this protein is stimulated by light and the protein has not been detected in roots. It has been localized in chloroplast stroma.

Molecular Weight: expected: 57 kDa, apparent: 50.5 kDa

UniProt: Q6STH5