Psbd antibody (C-Term)

Catalog No. ABIN125883

Product Details anti-Psbd Antibody

Target: Psbd (D2 Protein of Psii (Psbd))

Binding Specificity: C-Term

Reactivity: Pisum sativum, Thalassiosira pseudonana, Arabidopsis thaliana, Chlamydomonas reinhardtii, Green bean, Hordeum vulgare subsp. spontaneum (Wild barley) (Hordeum spontaneum), Oryza sativa

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Specificity: Perfect across all full-length PsbD sequences from higherplants, lowerplants, cyanobacteria and unicellular algae except: -minorsubstitutions in some Prochlorococcus & Dinoflagellatesequences. The antibody should still work against these taxa,but it has not been tested yet. This antibody does not detectPsbA protein (D1).

Cross-Reactivity (Details): Peptide target chosen from the N-terminal domain, nearly fully conserved within3-4 NtrA-related proteins from Arabidopsis thalina and with NtrC related proteinsfrom Chlamydomonas reinhardtii and Synechococcus sp. 7942 and othercyanobacteria .

Characteristics: Expected / apparent Molecular Weight of the Antigene: 39.4 / 28-30 kDa

Purification: serum

Immunogen: KLH-comjugated synthetic peptide derived from the C-terminal of known PsbD sequences including Arabidopsis pumila A4QJS8 , Hordeum vulgare P11849, Chlamydomonas reinhardtii P06007, Synechococcus sp. PCC 7002 P20898

Application Details

Application Notes: Recommended Dilution: 1 : 5000 with standard ECL (WB).

Comment:

There is a confirmed cross-reaction with TLA1 protein in Chlamydomonas reinhardtii.For samples with a very low PSII content theremight be detection problems independent of the antibody. PSII proteins can vary in level depending upon liquid culture conditions. When the cells are in a stationary phase PSII content can drop to a very low level.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-Psbd antibody (ABIN125883)

Mou, Zhang, Dong, Fan, Xu, Cao, Xu, Wang, Ye: "Photoprotection in the green tidal alga Ulva prolifera: role of LHCSR and PsbS proteins in response to high light stress." in: Plant biology (Stuttgart, Germany), (2013) (PubMed).

Signorelli, Casaretto, Sainz, Díaz, Monza, Borsani: "Antioxidant and photosystem II responses contribute to explain the drought-heat contrasting tolerance of two forage legumes." in: Plant physiology and biochemistry : PPB / Société française de physiologie végétale, Vol. 70, pp. 195-203, (2013) (PubMed).

Yamatani, Sato, Masuda, Kato, Morita, Fukunaga, Nagamura, Nishimura, Sakamoto, Tanaka, Kusaba: "NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll - protein complexes during leaf senescence." in: The Plant journal : for cell and molecular biology, Vol. 74, Issue 4, pp. 652-62, (2013) (PubMed).

Latijnhouwers, Xu, Muoller: "Arabidopsis stromal 70-kDa heat shock proteins are essential for chloroplast development." in: Planta, (2010) (PubMed).

Perreault, Dionne, Didur, Juneau, Popovic: "Effect of cadmium on photosystem II activity in Chlamydomonas reinhardtii: alteration of O-J-I-P fluorescence transients indicating the change of apparent activation energies within photosystem II." in: Photosynthesis research, (2010) (PubMed).

Target Details for Psbd

Target: Psbd (D2 Protein of Psii (Psbd))

Abstract: Psbd Products

Background: AGI Code: ATCG00270
D2 protein (PsbD) forms the reaction core of PSII (Photosystem II) as a heterodimer with the D1 protein (PsbA). PsbD is homologous to the D1 protein, with slightly higher molecular mass of about 39,5 kDa. Accumulation of D2 protein is an important step in the assemply of the PSII reaction centre complex.

Molecular Weight: expected: 39.4 kDa, apparent: 28-30 kDa

UniProt: P20898, A4QJS8, P06007, P11849