CD5 antibody

Catalog No. ABIN125749

Product Details anti-CD5 Antibody

Target: CD5

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD5 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The mouse monoclonal antibody CRIS1 reacts with an extracellular epitope of CD5, a 67 kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).

Cross-Reactivity (Details): Human, Other species Not tested

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: stimulated human leukocytes

Clone: CRIS1

Isotype: IgG2a

Application Details

Application Notes: ELISA: The antibody CRIS1 can be used in the sandwich ELISA as the detection antibody in pair with the capture antibody MEM-32
Flow cytometry: Recommended dilution: 1 μg/mL, positive control: peripheral blood lymphocytes (PBL), Jurkat human leukemia T-cell line, HPB human leukemia T-cell line, MOLT-4 human leukemia T-cell line.
Western blotting: Laurylmaltoside lysing buffer, non-reducing conditions, recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD5 antibody (ABIN125749)

Guarné, Bravo, Calvo, Lozano, Vives, Fita: "Conformation of the hypervariable region L3 without the key proline residue." in: Protein science : a publication of the Protein Society, Vol. 5, Issue 1, pp. 167-9, (1996) (PubMed).

Alberola-Ila, Places, Cantrell, Vives, Lozano: "Intracellular events involved in CD5-induced human T cell activation and proliferation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 5, pp. 1287-93, (1992) (PubMed).

Arrizabalaga, Mirapeix, Darnell, Torras, Revert: "Cellular immunity analysis using monoclonal antibodies in human glomerulonephritis." in: Nephron, Vol. 53, Issue 1, pp. 41-9, (1989) (PubMed).

Target Details for CD5

Target: CD5

Alternative Name: CD5 (CD5 Products)

Synonyms: CD5 antibody, Ly-1 antibody, Ly-12 antibody, Ly-A antibody, Lyt-1 antibody, LEU1 antibody, T1 antibody, CD5 molecule antibody, CD5 antigen antibody, CD5 molecule like antibody, Cd5 molecule antibody, CD5 antibody, Cd5 antibody, CD5L antibody

Background: CD5 Molecule,CD5 antigen (T1, 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells it seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (rheumatoid arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.,T1, LEU1

Gene ID: 921

UniProt: P06127