GST-Tag antibody

Catalog No. ABIN125746

Product Details anti-GST-Tag Antibody

Target: GST-Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This GST-Tag antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificity: The antibody S-tag-05 is a mouse monoclonal antibody with high affinity to the glutathione-S-transferase (GST) from Schistosoma japonicum. This purified antibod is suitable for detecting fusion proteins containing the GST-Tag sequence expressed in E. coli, yeast, mammalian, and in vitro transcription/translation systems.

Cross-Reactivity (Details): Recognizes fusion proteins in all species

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: GST fusion protein

Clone: S-tag-05

Isotype: IgG2b

Application Details

Application Notes: Western blotting: Recommended dilution: 1 μg/mL, positive control: GST fusion protein, reducing conditions.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for GST-Tag

Target: GST-Tag

Alternative Name: GST tag (GST-Tag Products)

Target Type: Tag

Background: The GST (glutathione-S-transferase) is a 26 kDa protein encoded by the parasite Schistosoma japonicum, easily purified by affinity chromatography on immobilized glutathione. GST is utilized as a fusion protein with foreign proteins in a range of prokaryotic expression vectors, including the pGEX family of vectors.