HLA-DR antibody
(1 reference)-
- Target See all HLA-DR products
- HLA-DR
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This HLA-DR antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)
- Specificity
- The antibody MEM-12 recognizes a common extracellular epitope on human HLA-DR which is dependent on the association of alpha and beta chains. DR is the isotype of human MHC Class II molecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages).
- Cross-Reactivity (Details)
- Human
- Purification
- Purified by protein-A affinity chromatography.
- Purity
- > 95 % (by SDS-PAGE)
- Immunogen
- thymocyte membrane
- Clone
- MEM-12
- Isotype
- IgG1
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anti-HLA-DR (HLA-DR) antibody (FITC)
HLA-DR Reactivity: Human FACS Host: Mouse Monoclonal MEM-12 FITC
anti-HLA-DR (HLA-DR) antibodyHLA-DR Reactivity: Human FACS, WB, IHC, ISt, StM Host: Mouse Monoclonal TAL 1B5 unconjugated
anti-HLA-DR (HLA-DR) antibodyHLA-DR Reactivity: Human, Dog, Non-Human Primate FACS, WB, IP, IHC (fro), IHC (p), ICC Host: Mouse Monoclonal L243 unconjugated
anti-HLA-DR (HLA-DR) antibody (PE)HLA-DR Reactivity: Human, Dog, Non-Human Primate FACS Host: Mouse Monoclonal L243 PE
anti-HLA-DR (HLA-DR) antibodyHLA-DR Reactivity: Human FACS, WB, IHC, IF, ISt, StM Host: Mouse Monoclonal SPM289 unconjugated
anti-HLA-DR (HLA-DR) antibodyHLA-DR Reactivity: Human FACS, IP Host: Mouse Monoclonal HL-39 unconjugated
anti-HLA-DR (HLA-DR) antibodyHLA-DR Reactivity: Human, Dog, Non-Human Primate FACS, WB, IP, IHC (fro), IHC (p), ICC, Func Host: Mouse Monoclonal L243 unconjugated
anti-HLA-DR (HLA-DR) antibody (PE)HLA-DR Reactivity: Human FACS Host: Mouse Monoclonal MEM-12 PE
anti-HLA-DR (HLA-DR) antibody (APC)HLA-DR Reactivity: Human FACS Host: Mouse Monoclonal MEM-12 APC
anti-HLA-DR (HLA-DR) antibody (PerCP)HLA-DR Reactivity: Human FACS Host: Mouse Monoclonal MEM-12 PerCP
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- Application Notes
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Flow cytometry: Recommended dilution: 2 μg/mL, positive control: B-lymphocytes, monocytes, DAUDI cell line, RAJI cell line.
Western blotting and Immunoprecipitation: Positive control: B-lymphocytes, monocytes, DAUDI cell line, RAJI cell line, non-reducing conditions. - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
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-
: "Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens." in: Tissue antigens, Vol. 28, Issue 5, pp. 288-97, (1987) (PubMed).
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: "Characterization of seven new monoclonal antibodies against human DR, DR + DP and DQ1 + DQ3 antigens." in: Tissue antigens, Vol. 28, Issue 5, pp. 288-97, (1987) (PubMed).
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- Target
- HLA-DR
- Alternative Name
- HLA-DR (HLA-DR Products)
- Background
- HLA-DR, a member of MHC class II glycoproteins, that bind intracellularly processed peptides and present them to the Th cells, is composed of 36 kDa alpha chain and 27 kDa beta chain, both anchored in the plasma membrane. Together with other MHC II molecules HLA-DR plays a central role in the immune system.
- Pathways
- Human Leukocyte Antigen (HLA) in Adaptive Immune Response
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