CD90.2 / Thy-1.2 antibody

Catalog No. ABIN125729

Product Details anti-CD90.2 / Thy-1.2 Antibody

Target: CD90.2 / Thy-1.2

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD90.2 / Thy-1.2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Functional Studies (Func), Immunocytochemistry (ICC)

Specificity: The mouse monoclonal antibody 1aG4/C5 recognizes alloantigen Thy-1.2 of mouse Thy-1 (CD90), it does not cross-react with Thy-1.1 alloantigen. Thy-1 is an 18-35 kDa cell surface GPI anchored glycoprotein (immunoglobulin superfamily), in mouse it is abundant on thymocytes and peripheral T lymphocytes.

No Cross-Reactivity: Human, Rat

Cross-Reactivity (Details): Mouse

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: thymocytes from C3H/Di mice (Thy-1.2 positive)

Clone: 1aG4-C5

Isotype: IgG3

Application Details

Application Notes: Functional application: The bound of antibody 1aG4/C5 to the transfected mast cells induces the activation processes which are similar, but not identical, to the antigen activation of IgE stimulated cells.
Immunoprecipitation: The antibody 1aG4/C5 precipitates complexes with Lyn protein tyrosine kinase.
Flow cytometry: Recommended dilution: 1 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Preservative: Azide free

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD90.2 / Thy-1.2 antibody (ABIN125729)

Bradley, Ramirez, Hagood: "Roles and regulation of Thy-1, a context-dependent modulator of cell phenotype." in: BioFactors (Oxford, England), Vol. 35, Issue 3, pp. 258-65, (2009) (PubMed).

Heneberg, Lebduska, Dráberová, Korb, Dráber: "Topography of plasma membrane microdomains and its consequences for mast cell signaling." in: European journal of immunology, Vol. 36, Issue 10, pp. 2795-806, (2006) (PubMed).

Dráberová, Dráber: "Thy-1 glycoprotein and src-like protein-tyrosine kinase p53/p56lyn are associated in large detergent-resistant complexes in rat basophilic leukemia cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 8, pp. 3611-5, (1993) (PubMed).

Dráber, Zikán, Vogtísková: "Establishment and characterization of permanent murine hybridomas secreting monoclonal anti-thy-1 antibodies." in: Journal of immunogenetics, Vol. 7, Issue 6, pp. 455-74, (1981) (PubMed).

Target Details for CD90.2 / Thy-1.2

Target: CD90.2 / Thy-1.2

Abstract: CD90.2 / Thy-1.2 Products

Synonyms: CD90 antibody, T25 antibody, Thy-1 antibody, Thy-1.2 antibody, Thy1.1 antibody, Thy1.2 antibody, thymus cell antigen 1, theta antibody, Thy1 antibody

Background: Thy-1.2 cell surface antigen,CD90 (Thy-1) is an 18-35 kDa GPI-anchored plasma membrane glycoprotein expressed in many cell types, such as in hematopoietic cells and neurons, connective tissues, various fibroblast and stromal cell lines, tumor endothelial cell lines and other. In the mouse, CD90 is expressed mainly on thymocytes and peripheral T lymphocytes. It is involved in T cell activation, cellular adhesion, proliferation and migration, neurite outgrowth, wound healing, apoptosis, and fibrosis. CD90 participates in multiple signaling cascades and its effects are tissue- and cell type-specific. It often functions as an important regulator of cell-cell and cell-matrix interactions.,Thy-1.2

Gene ID: 21838

UniProt: P01831