TCR beta (Vbeta5.3-Related) antibody
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- Target
- TCR beta (Vbeta5.3-Related)
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
- Application
- Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Functional Studies (Func)
- Specificity
- The antibody MEM-262 recognizes an extracellular epitope on beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.
- Cross-Reactivity (Details)
- Other not tested, Human
- Purification
- Purified by protein-A affinity chromatography.
- Purity
- > 95 % (by SDS-PAGE)
- Immunogen
- Human thymoma cell line HPB-ALL.
- Clone
- MEM-262
- Isotype
- IgG2a
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- Application Notes
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Western blotting: This antibody uniquely recognizes denatured TCR beta chains under the conditions of Western blotting.
Functional application: This antibody activates T cells (V beta 5-related subset).
Flow cytometry: Recommended dilution: 1-4 μg/mL - Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4
- Preservative
- Azide free
- Handling Advice
- Do not freeze.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
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A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor." in: Immunology letters, Vol. 88, Issue 2, pp. 105-8, (2003) (PubMed).
: "
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A novel monoclonal reagent recognizing native and denatured Vbeta5.3-related chains of human T cell receptor." in: Immunology letters, Vol. 88, Issue 2, pp. 105-8, (2003) (PubMed).
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- Target
- TCR beta (Vbeta5.3-Related)
- Background
- TCRVB5.3-related
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