SIT1 antibody (PE)

Catalog No. ABIN125717

Product Details anti-SIT1 Antibody (PE)

Target: SIT1 (Signaling threshold Regulating Transmembrane Adaptor 1 (SIT1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIT1 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: The antibody SIT-01 reacts with an intracellular epitope of SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with murine SIT.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Bacterially produced recombinant intracellular fragment of human SIT.

Clone: SIT-01

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.

Comment:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-SIT1 antibody (PE) (ABIN125717)

Tedoldi, Paterson, Hansmann, Natkunam, Rüdiger, Angelisova, Du, Roberton, Roncador, Sanchez, Pozzobon, Masir, Barry, Pileri, Mason, Marafioti, Horejsí: "Transmembrane adaptor molecules: a new category of lymphoid-cell markers." in: Blood, Vol. 107, Issue 1, pp. 213-21, (2005) (PubMed).

Horejsí, Zhang, Schraven: "Transmembrane adaptor proteins: organizers of immunoreceptor signalling." in: Nature reviews. Immunology, Vol. 4, Issue 8, pp. 603-16, (2004) (PubMed).

Target Details for SIT1

Target: SIT1 (Signaling threshold Regulating Transmembrane Adaptor 1 (SIT1))

Alternative Name: SIT (SIT1 Products)

Synonyms: SIT1 antibody, RP11-331F9.5 antibody, SIT antibody, SIT-R antibody, Sit antibody, signaling threshold regulating transmembrane adaptor 1 antibody, suppression inducing transmembrane adaptor 1 antibody, SIT1 antibody, Sit1 antibody

Background: Signaling threshold regulating transmembrane adapt,SIT is a 21 kDa transmembrane adaptor protein expressed exclusively in lymphoid organs. It acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.,SIT1

Gene ID: 27240

UniProt: Q9Y3P8