Cytokeratin 19 antibody (Biotin)
(2 references)-
- Target See all Cytokeratin 19 (KRT19) Antibodies
- Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Cytokeratin 19 antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Specificity
- The mouse monoclonal antibody A53-B/A2 recognizes the rod domain of cytokeratin 19 (40 kDa), an intracellular antigen constituting intermmediate cytoskeleton filaments. Cytokeratin 19 is not expressed in hepatocytes, it is often co-expressed with cytokeratin 7.
- Cross-Reactivity (Details)
- Human
- Purification
- Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
- Immunogen
- MCF-7 human breast adenocarcinoma cell line
- Clone
- A53-B-A2
- Isotype
- IgG2a
- Top Product
- Discover our top product KRT19 Primary Antibody
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anti-Keratin 19 (KRT19) antibody
KRT19 Reactivity: Human WB, FACS, ELISA, IHC (p), IP, ICC Host: Mouse Monoclonal A53-B-A2 unconjugated
anti-Keratin 19 (KRT19) (AA 245-391) antibodyKRT19 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Keratin 19 (KRT19) (AA 245-391) antibodyKRT19 Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal 1# unconjugated
anti-Keratin 19 (KRT19) (AA 1-403) antibodyKRT19 Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Keratin 19 (KRT19) antibodyKRT19 Reactivity: Human WB, FACS, IHC (p), IP, ICC Host: Mouse Monoclonal BA-17 unconjugated
anti-Keratin 19 (KRT19) (AA 127-322) antibodyKRT19 Reactivity: Human WB, IHC, IP, ICC Host: Mouse Monoclonal D1 unconjugated
anti-Keratin 19 (KRT19) (AA 80-400) antibodyKRT19 Reactivity: Human WB, IHC, FACS, ELISA Host: Mouse Monoclonal 2A1G9 unconjugated
anti-Keratin 19 (KRT19) (AA 274-400) antibodyKRT19 Reactivity: Human WB, IHC, ELISA Host: Mouse Monoclonal 8H1A8 unconjugated
anti-Keratin 19 (KRT19) antibodyKRT19 Reactivity: Human, Mouse, Rat IHC, IF, FACS, StM Host: Mouse Monoclonal KRT19-800 unconjugated
anti-Keratin 19 (KRT19) antibodyKRT19 Reactivity: Human WB, IHC, IF, FACS, StM Host: Mouse Monoclonal KRT19-799 unconjugated
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- Application Notes
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Flow cytometry: Recommended dilution: 1-5 μg/mL, intracellular staining.
Western blotting: Recommended dilution: 1-2 μg/mL. - Comment
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The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
- Restrictions
- For Research Use only
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- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Do not freeze.
Avoid prolonged exposure to light. - Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
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: "Patterns of cytokeratin and vimentin expression in the human eye." in: Histochemistry, Vol. 89, Issue 4, pp. 369-77, (1988) (PubMed).
: "Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion." in: European journal of cancer & clinical oncology, Vol. 21, Issue 6, pp. 733-40, (1985) (PubMed).
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: "Patterns of cytokeratin and vimentin expression in the human eye." in: Histochemistry, Vol. 89, Issue 4, pp. 369-77, (1988) (PubMed).
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- Target
- Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))
- Alternative Name
- Cytokeratin 19 (KRT19 Products)
- Background
- Keratin 19,Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,K19, CK19, CYK19, KRT19, K1CS
- Gene ID
- 3880
- UniProt
- P08727
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