Immunohistochemistry with formalin fixed paraffin embedded tissues: 6 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, pH 7.7, containing 0.01 % sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-80 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
NTR1, a Neurotensin Receptor, activates a phosphatidylinositol-calcium second messenger system in the central and peripheral nervous systems. It mediates the functions of neurotensin, such as hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion. Neurotensin Receptor 1 expression is enhanced in pancreatic cancer and in chronic pancreatitis. Two differentially glycosylated forms of the NT1 receptor have been identified in rats, a 54- kDa form that is expressed from birth to adulthood, and a 52- kDa form only detected at 10 and 15 days postnatal. NTR1 has been reported in brain, pancreas, and small intestine. ESTs have been isolated from brain and colon libraries.Synonyms: High-affinity levocabastine-insensitive neurotensin, NT-R-1, NTR1, NTRR, NTSR1