GFP antibody

Catalog No. ABIN121945

Product Details anti-GFP Antibody

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Specificity: The antibody reacts specifically with GFP, EGFP, EYFP fusion proteins in all species.

Purification: Affinity Chromatography on Protein A

Immunogen: EGFP, a native full-length protein

Application Details

Application Notes: Immunoprecipitation (10-20 μg/sample). Immunocytochemistry (1-3 μg/mL). Western Blotting (0.5-1.5 μg/mL). Positive control: transfected cells. Negative control: non-transfected cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 15 mM Sodium Azide, 0.2 % (w/v) high-grade BSA (protease free)

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

References for anti-GFP antibody (ABIN121945)

Prescher, Baumgärtel, Ivanchenko, Torrano, Bräuchle, Müller, Lamb: "Super-resolution imaging of ESCRT-proteins at HIV-1 assembly sites." in: PLoS pathogens, Vol. 11, Issue 2, pp. e1004677, (2016) (PubMed).

Gentzel, Schille, Rauschenberger, Schambony: "Distinct functionality of dishevelled isoforms on Ca2+/calmodulin-dependent protein kinase 2 (CamKII) in Xenopus gastrulation." in: Molecular biology of the cell, Vol. 26, Issue 5, pp. 966-77, (2015) (PubMed).

Ling, Wong, Li, Chan, Boukamp, Liu: "CCHCR1 interacts with EDC4, suggesting its localization in P-bodies." in: Experimental cell research, Vol. 327, Issue 1, pp. 12-23, (2014) (PubMed).

Savard, Barbaz, Bélanger, Müller-Esterl, Bkaily, Dorléans-Juste, Coté, Bovenzi, Gobeil: "Expression of endogenous nuclear bradykinin B2 receptors mediating signaling in immediate early gene activation." in: Journal of cellular physiology, Vol. 216, Issue 1, pp. 234-44, (2008) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP (GFP Products)

Synonyms: green fluorescent protein antibody, gfp antibody

Background: Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (509 nm) when excited (excitation by Blue or UV light, absorption peak at 395 nm). GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. To increase a fluorescence intensity of GFP, chromophore mutations have been created. The Enhanced GFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties. Antibodies raised against full-length GFP variants should also detect other variants of the protein.Synonyms: GFP-Tag, Green fluorescent protein

UniProt: P42212