Caspase 7 antibody (C-Term)

Catalog No. ABIN121893

Product Details anti-Caspase 7 Antibody

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 7 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody recognises an epitope within the C-terminal region (CT) of Caspase-7, a 35 kDa cysteine protease, otherwise known as ICE-like Apoptotic Protease 3 (ICE-LAP3) Caspase-7.

Purification: Affinity Chromatography

Immunogen: A 16 amino acid peptide from human Caspase-7 carboxy-terminus

Isotype: IgG

Application Details

Application Notes: Western Blot: 1.0 μg/mL - 2.0 μg/mL. Immunohistochemistry on paraffin sections: 2.0 μg/mL, this product does not require protein digestion pre-treatment of paraffin sections eg. Trypsin or pronase prior to staing, this product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Recommended Positive Control: Skeletal muscle tissue.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.02 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for Caspase 7

Target: Caspase 7 (CASP7) (Caspase 7, Apoptosis-Related Cysteine Peptidase (CASP7))

Alternative Name: Caspase-7 (CASP7 Products)

Background: ICE-LAP3 is a member of the ICE/Ced-3 subfamily, is an executioner caspase which undergoes proteolytic cleavage of its precursor to form active p12 and p20 subunits. Evidence that activation of Caspase-7 occurs during cell death induced by the cytokine death receptors Fas/APO-1 and the receptor of tumour necrosis factor (TNFR-1), coupled with the fact that granzyme-B activated Caspase-7 cleaves the nuclear enzyme poly (ADPribose) polymerase (PARP), suggests an important role for Caspase-7 in both cytokine-mediated and Granzyme-B mediated apoptosis pathways.Synonyms: Apoptotic protease Mch-3, CASP-7, CASP7, CMH-1, ICE-like apoptotic protease 3, MCH3

UniProt: P55210

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity